猴头菌多糖的理化性质及抗胃溃疡活性研究

Physicochemical properties and anti-gastric ulcer activity of Hericium erinaceus polysaccharide

以猴头菌的菌丝提取物为原料,采用水煎醇沉,中孔纤维柱及DEAE-Sephadex A-50凝胶柱纯化的方法制备了猴头菌多糖,并命名为HEP-1。通过HEP-1对小鼠无水乙醇诱导胃溃疡模型及MC细胞模型的影响,分别测定溃疡面积、计算溃疡抑制率及MTT法测定细胞抑制率,研究该多糖的抗胃溃疡活性。结果HEP-1在水中溶解性较好,通过苯酚-硫酸测得HEP-1中性糖含量为95.2%、间羟基联苯法测得酸性糖含量为零、BCA试剂盒法测得蛋白质含量为5.5%;PMP柱前衍生化,对单糖的组成进行了分析研究表明该多糖由甘露糖、葡萄糖及半乳糖组成,其组成比例为甘露糖:葡萄糖:半乳糖=4:94:2;该多糖对小鼠无水乙醇诱导的胃溃疡具有很好的抑制作用;并能够抑制MC细胞的生长。说明该猴头菌多糖具有良好的抗胃溃疡活性。

In the study, Hercium erinaceus mycelium was used as raw material, one polysaccharide named HEP-1 was isolated by water extracted, precipitated with ethanol, hollow fibre ultrafiltration and ion-exchange chromatography. The anti-gastriculcer activity was evaluated in experimental systems using ethanol-induced model in mice and MC cells to identify. The area of ulcer, inhibition of ulcer and the inhibitin of the MC cells were measurece respctivrly. Total carbohydrate, uronic acid and protein contents of HEP-1 were quantified by the phenol-sulphuric acid, m-hydroxydiphenyl and Bradford methods, respectively, using glucose （glu）, glucuronic acid （gluA）, and bovine serum albumin （BSA） as standards, respectively. Carbohydrate content was 5.5%, protein content was 95.2%, sugar components was Man：glu：gal=4：94：2. The results indicated that HEP-1 showed significant inhibition of ethanol-induced gastric ulcers in mice, and also inhibited the growth of MC cells.