小麦粉中偶氮甲酰胺检测方法研究

Determination of azodicarbonamide in wheat flour

建立小麦粉中偶氮甲酰胺含量的液相色谱分析方法。小麦粉中偶氮甲酰胺用N，N-二甲基甲酰胺振荡提取，离心过滤后液相色谱仪定量测定。该方法在0-50μg／mL范围内线性关系良好，相关系数0．9997；最小检测限为30．2ng／mL，方法回收率在95．3％～101％之间，相对标准偏差小于1．3％。该方法前处理简单、快速，定量准确，灵敏度高，可用于实验室检测小麦粉中偶氮甲酰胺含量。

A method for the determination of azodicarbonamide in wheat flour by high performance liqide chromatography was developed. Azodicarbonamide was extracted by N,N-dimethylformamide and analyzed by HPLC with external standard method. The linear range of the method was 0-50 μg/mL（r=0.9997）. The detection limit of azodicarbonamide was 30.2 ng/mL, The recovery of azodicarbonamide was 95.3%-101%. The RSD was 1.3%. It is a simple, rapid, accuracy and featuring high sensetivity method that could be applied in the lab.