肝癌组织中HuR和HIF-1α的表达及其相关性

Expression and correlation of HuR and HIF-1α in hepatocellular carcinoma

目的观察人抗原R(HuR)和缺氧诱导因子(HIF)-1α在肝癌组织中的表达变化,并探讨其相关性。方法取48例肝癌组织及癌旁组织标本,采用免疫组化染色法检测肝癌组织及癌旁组织中HuR和HIF-1α蛋白的表达,采用荧光定量PCR法检测肝癌组织中HIF-1αmRNA的表达。结果肝癌组织中HuR和HIF-1α蛋白阳性表达率分别为67%和58%,显著高于癌旁组织的19%和10%(P均<0.01)。肝癌组织中HuR与HIF-1α蛋白的表达呈正相关(r=0.723,P<0.01)。HuR蛋白高表达者中HIF-1αmRNA的表达较HuR蛋白低表达者显著上调(P<0.05)。结论肝癌组织中HuR和HIF-1α蛋白表达上调且呈正相关关系,HuR可能增强HIF-1αmRNA的稳定性,从而促进其表达。

Objective To investigate the expression and correlation of human antigen R （ HuR） and hypoxia-induc-ible factor （HIF）-1αin hepatocellular carcinoma （HCC） tissues.Methods Forty-eight cases of HCC tissue and adjacent tissue samples were collected from patients undergoing surgical resection .The expression of HuR and HIF-1αprotein in the HCC and adjacent tissues was detected by immunohistochemical staining .The mRNA expression of HIF-1αin the HCC tis-sues was detected by fluorescent quantitative PCR .Results The positive expression rates of HuR and HIF-1αprotein in the HCC tissues were 67%and 58%, respectively .These rates were significantly higher than the positive expression rates of HuR and HIF-1αprotein （19%and 10 %） in the adjacent tissues （ all P〈0.01） .The expression of HuR protein was positively correlated with the expression of HIF-1αprotein in the HCC tissues （r=0.723, P〈0.01）.The mRNA expres-sion of HIF-1αmRNA in the group with high expression of HuR protein was significantly up-regulated as compared with that of the group with low expression of HuR protein （P〈0.05）.Conclusions The expression of HuR and HIF-1αprotein was up-regulated and positively correlated with each other in the HCC tissues .HuR may enhance the stability of HIF-1αmRNA, and thus increase the expression of HIF-1αprotein.