早期自然流产患者底蜕膜绒毛外滋养细胞中IGFBP7、VEGF的表达变化及意义被引量：1

下载PDF

导出

摘要目的观察早期自然流产患者底蜕膜绒毛外滋养细胞(EVT)中胰岛素样生长因子结合蛋白7(IGFBP7)、血管内皮生长因子(VEGF)的表达变化,并探讨其意义。方法早期自然流产患者20例(观察组),人工流产患者20例(对照组),采用免疫组化法检测两组患者EVT中IGFBP7、VEGF。结果观察组及对照组IGFBP7免疫染色评分(IRS)分别为(10.75±1.28)、(8.63±1.06)分,VEGF IRS分别为(5.25±1.98)、(7.75±2.25)分,两组比较,P均<0.05。Pearson相关分析显示,观察组IGFBP7、VEGF表达无相关性(r=-0.32,P=0.44)。结论早期自然流产患者EVT中IGFBP7表达升高,VEGF表达降低,两指标表达异常可能与早期自然流产的发生有关。

作者杨倩卢丽莉孟丽婵宋春红

机构地区石家庄市第四医院石家庄市第三医院

出处《山东医药》 CAS 北大核心 2015年第23期68-70,共3页 Shandong Medical Journal

基金石家庄市科技局立项课题(131460543)

关键词自然流产绒毛外滋养细胞胰岛素样生长因子结合蛋白7 血管内皮生长因子

分类号 R714.21 [医药卫生—妇产科学]

引文网络
相关文献

参考文献13

1Huppertz B. Extravillous trophoblast: proliferation and invasion during pregnancy[J]. Pathologica, 2003,95(5) :231-232.
2Liu ZK, Liu HY, Fang WN, et al. Insulin-like growth factor binding protein 7 modulates estrogen-induced trophoblast proliferation and invasion in HTR-8 andJEG-3 cells[J]. Cell Biochem Biophys, 2012,63(1) :73-84.
3Athanassiades A, Hamilton GS, Lala PK. Vascular endothelial growth factor stimulates proliferation but not migration or invasiveness in human extravillous trophoblast[J]. BioI Reprod, 1998,59 (3) :643-654.
4Tamura K, Hashimoto K, Suzuki K, et al. Insulin-like growth factor binding protein-7 (IGFBP7) blocks vascular endothelial cell growth factor( VEGF) -induced angiogenesis in human vascular endothelial cells[J]. EurJ Pharmacol, 2009,610(1-3) :61-67.
5Tamura K, Y oshie M, Hashimoto K, et al. Inhibitory effect of insulin-like growth factor-binding protein-7 (IGFBP7) on in vitro angiogenesis of vascular endothelial cells in the rat corpus luteum[J].J Reprod Dev, 2014,60(6) :447-453.
6Detre S, SaclaniJotti G, Dowsett M. A" quickscore" method for immunohistochemical semiquantitation , validation for oestrogen receptor in breast oarcinomasJ J].J Clin Pathol, 1995,48(9) :876- 878.
7RebeccaN.人类胎盘病理学手册[M].天津:天津科技翻译出版公司,2008:116-118.
8K weider N, Fragoulis A, Rosen C, et al. Interplay between vascular endothelial growth factor (VEGF) and nuclear factor erythroid 2-related factor-2 (Nrf2) : implications for preeclampsia]J J.J BioI Chern, 2011 ,286(50) :42863-42872.
9夏红飞,孙敬,杨颖,彭景楩.胰岛素样生长因子结合蛋白-7′在大鼠胚胎植入过程中的差异表达与调节[J].生物化学与生物物理进展,2007,34(3):267-274. 被引量：4
10Liu ZK, Wang RC, Han BC, et al. A novel role of IGFBP7 in mouse uterus: regulating uterine receptivity through ThllTh2 lymphocyte balance and decidualieationJ J]. PLoS One, 2012,7(9): e45224.

二级参考文献38

1孙敬,陈宏,杨颖,夏红飞,彭景楩.植入前期与分娩前期子宫差别表达基因筛选及表达[J].生物化学与生物物理进展,2005,32(2):133-139. 被引量：1
2Kim NK, Choi MJ, Chung HM, Sohn TJ, Hwang SG, Oh D, & Lee HH. Increased expression and localization of a serine protease inhibitor, protease nexin-1(PN-1) ,in the ovary and uterus during implantation in rat. Thromb Res, 2001, 103(2) : 135- 42.
3Xu P, Wang YL, Zhu SJ, Luo SY, Piao YS & Zhuang LZ. Expression of matrix metalloproteinase-2,-9,and-14, tissue inhibitors of metalloproteinase-1, and matrix proteins in human placenta during the first trimester. Biol Reprod, 2000, 62(4): 988-94.
4Tanaka SS, Togooka Y, Sato H, Seiki M, Tojo H &Tachi C. Expression and location of membrane type matrix metalloproteinase-1 ( MT1-MMP ) in trophoblast cells of cultured mouse blastocysts and ectoplacental cones. Placenta, 1998, 9(1):41-8.
5Hurskainen T, Seiki M, Apte SS, Syrjakallio-Ylitalo M, Sorsa T, Oikarinen A & Autio-Harmainen H. Production of membrane-type matrix metallproteinase(MT-MMP-1) in early human placenta. A possible role in placental implantation? J Histochem Cytochem,1998,46(2) : 221-9.
6Whiteside EJ, Jackson MM, Herington AC, Edwards DR & Harvey MB. Matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 are key regulators of extracellular matrix degradation by mouse embryos.Biol Reprod, 2001,64(5):1 331-7.
7Oh J, Takahashi R, Kondo S, Mizoguchi A, Adachi E, Sasahara RM, Nishimura S, Imamura Y, Kitayama H, Alexander DB, Ide C, Horan TP, Arakawa T,Yoshida H, Nishikawa SI, Itoh Y, Seiki M & Itohara S. The membrane-achored MMP inhibitor RECK is a key regulator of extraeellular matrix integrity and angiogenesis. Cell, 2001, 107(6) :789-800.
8Raga F, Casan EM, Wen Y, Huang HY, Bonilla-Mu-soles F & Polan ML. Independent regulation of matrix metalloproteinase-9, tissue inhibitor of metallopro-teinase-1 (TIMP-1) ,and TIMP-3 in human endometrial stromal cells by gonadotropin-relesasing hormone:Implications in early human implantation. J Clin Endocrinol & Metab, 1999, 84(2) :636-42.
9Murray SC, Keeble SC, Muse KN & Curry TE. Regulation of granulose cell-derived ovarian metalloproteinase inhibitor (s) by prolactin. J Reprod Ferti,1996, 1 107(1): 103-8.
10Zhang J, Hampton AL, Nie G & Salamonsen LA.Progesterone inhibits activation of latent matrix metalloproteinase(MMP)-2 by membrane-type 1 MMP:enzymes coordinately expressed in human endometrium.Biol Reprod, 2000, 62(1) :85-94.