肥厚型心肌病心肌组织TGFβ异构体表达及意义

EXPRESSION AND ITS SIGNIFICANCE OF TGFβ IN HYPERTROPHIC CARDIOMYOPATHY

目的研究转化生长因子β（TGFβ）异构体在肥厚型心肌病病人心肌组织中的表达，以探讨TGFβ异构体与肥厚型心肌病心肌纤维化的关系，为法医学诊断肥厚型心肌病提供客观指标。方法采用免疫组化方法，检测TGFβ1、TGFβ2、TGFβ3在56例肥厚型心肌病病人及18例对照组心肌组织中的表达水平，并使用图像分析系统进行结果判断。结果TGFβ1、TGFβ2、TGFβ3在肥厚型心肌病病人心肌中的表达均高于对照者，差异有显著性（t=20．18～28．93，P〈0．05）。结论TFGβ异构体在肥厚型心肌病心肌纤维化的发生发展中具有重要作用，是法医诊断肥厚型心肌病的客观指标。

Objective To study the expression of transforming growth factor-β （TGFβ） isomer in cardiac muscle of pa tients with hypertrophic cardiomyopathy （HC）, explore the relationship between TGFβ3 isomer and myocardial fibrosis in HC, and provide objective indicators for forensic diagnosis of the disease. Methods Employing immunohistochemical method, expressions of TGFβ1, TGFβ2 and TGFβ3 in tissue of cardiac muscle of 56 HC patients and 18 controls were detected, the results were judged using image analysis system. ; Results The expressions of TGFβ1, .TGFβ2 and TGFβ3 in HC patients were higher than healthy controls, the difference were statistically significant （t=20.18--28.93,P〈0.05）. Conclusion TGFβ isomer plays an important role in the occurrence and development of myocardial fibrosis in hypertrophic eardiomyopathy, which is an objective indicator for medicolegal identification of this condition.