冠心病患者外周血单核细胞PTX-3与血清MMP-9和hs-CRP的相关性研究

Correlation between peripheral blood mononuclear cell PTX-3 and serum MMP-9 and hs-CRP in patients with coronary heart disease

目的探讨冠状动脉粥样硬化性心脏病(CHD)患者外周血单核细胞五聚蛋白-3(PTX-3)的表达及其与血清基质金属蛋白酶-9(MMP-9)、超敏C反应蛋白(hs-CRP)的相关性。方法选择CHD患者132例,其中不稳定型心绞痛(UAP)47例(UAP组),急性心肌梗死(AMI)43例(AMI组),稳定型心绞痛(SAP)42例(SAP组);另选同期相匹配的健康体检者33例作为对照组。采用酶联免疫吸附法(ELISA)分别测定各组受试者外周血单核细胞PTX-3及血清MMP-9水平,采用免疫比浊法测定hs-CRP水平,并采用pearson相关性分析法分析外周血单核细胞PTX-3、血清MMP-9水平及hs-CRP三者之间的相关性。结果 UAP组和AMI组外周血单核细胞PTX-3和血清MMP-9、hs-CRP水平均高于SAP组和对照组,差异均具有统计学意义(P<0.05),且AMI组中3种炎症因子水平均高于UAP组,差异均具有统计学意义(P<0.05);SAP组与对照组3种炎症因子水平比较,差异均无统计学意义(P>0.05)。Pearson相关性分析显示,外周血单核细胞PTX-3与血清MMP-9、hs-CRP及血清MMP-9与hs-CRP之间呈正相关[r=0.427(P<0.01),0.244(P<0.05),0.197(P<0.05)]。结论 CHD受试者和健康受试者外周血单核细胞均可检测出PTX-3的存在,并且随着病情凶险程度的增加PTX-3和MMP-9、hs-CRP的水平逐渐升高;3种炎症因子之间呈正相关性,3种炎症因子水平可以作为预测CHD严重程度的指标。

Objective To observe the levels of peripheral blood mononuclear cell （PBMC） Pentraxin 3 （PTX-3） expression, and to study the relationship among PBMC PTX-3 expression levels, serum matrix metallo pretein- ases 9 （MMP-9） and serum high-sensitivity C-reactive protein （hs-CRP） levels in the patients with coronary heart disease. Methods A total of 132 patients of CHD were selected as experimental group, including 47 cases of trustable angina pectioris （UAP group）, 42 cases of stable angina pectoris （SAP group） and 43 cases of acute myocardial infarction （AMI group）. Besides, 33 healthy individuals for physical examination were selected as the healthy control group. The levels of peripheral blood mononuclear cells PTX-3 and serum MMP-9 were detected by ELISA. The levels of serum hs-CRP were detected by immunoturbidimetric assay. The correlations of the three were analyzed by using pearson eor- relation analysis. Results The levels of PBMC PTX-3, serum MMP-9 and serum hs-CRP in UAP group and AMI group were significantly higher than SAP group and control group （P〈0.05）. The levels of PBMC PTX-3, serum MMP-9 and serum hs-CRP in AMI group were significantly higher than those in UAP group （P〈0.05）. PBMC PTX-3, serum MMP-9 and serum hs-CRP in SAP group and eontrol group showed no statistically significant differenee （P〉 0.05）. There were positive correlations of PBMC PTX-3 with serum MMP-9, serum hs-CRP and serum MMP-9 with hs-CRP （r=0.427, P〈0.01; r=0.244, P〈0.05; r=42.197, P〈0.05, respectively）. Conclusion The levels of PBMCPTX-3, serum MMP-9 and serum hs-CRP increased as the deterioration of the disease. There is a positive correlation between the three kinds of inflammatory factors, and their levels can be used as a prediction index for the severity of CHD.