急性脑梗死外周血中NOX2、SOD水平变化的临床意义

Clinical significance of peripheral blood NOX2,SOD changes in acute cerebral infarction

目的：探讨外周血 NOX2、SOD 水平与急性脑梗死的关系。方法收集98例急性脑梗死患者，采用 ELISA 法以及 WST-1还原法分别测定急性脑梗死患者患病后24 h、3 d、7 d、14 d 以及对照组 SOD、NOX2在外周血的水平，并与91例健康者进行比较。结果脑梗死组外周血 SOD 值表达于发病后24 h 内降低显著多于对照组（P 〈0.01），至3-7 d 达到高峰（P 〈0.01），然后在发病后14 d 逐渐升高，水平仍低于对照组，但差异无统计学意义（P〉0.05）；脑梗死组 NOX2在发病后24 h 即明显升高（P 〈0.01），至3-7 d 达到最高值（P 〈0.01），之后又缓慢下降，至发病后14 d 接近正常。进一步将脑梗死分为轻、中、重三组，发现发病7 d 时重度、中度脑梗死组 NOX2、SOD 的水平与轻度脑梗死组、对照组比较，差异均有统计学意义（P 〈0.05）。结论急性脑梗死患者外周血 NOX2与 SOD 水平存在着动态变化，与脑梗死的严重程度有关，其浓度水平对急性脑梗死的病情变化及治疗具有重要的价值。

Objective To explore the relationship of acute cerebral infarction and peripheral blood NOX2 and SOD levels. Methods Ninety-eight cases of acute cerebral infarction patients were selected as the ob-servation group,and 91 cases of healthy individuals（in physical examination）were chosen as control group.The peripheral blood NOX2 and SOD levels in patients with acute cerebral infarction and in objects of the control group were measured 24 hours,3 days,7 days,14 days after disease attack were measured by using WST-1 re-duction method and ELISA method,respectively. Results Patients of the cerebral infarction group had more significantly decreasing peripheral blood SOD expression than the control group within 24 hours（P 〈0.01 ）, reached to the peak from day 3 to 7 （P 〈0.01）,and elevated gradually on day 14 after disease attack,SOD lev-el of the cerebral infarction group was still lower than the control group,but there was not statistically signifi-cant difference （P 〉 0.05 ）.The NOX2 value of cerebral infarction group increased significantly within 24 hours after disease attack （P 〈0.01）,while the value reached the peak from day 3 to 7 （P 〈0.01）,and then slowly declined,decreasing to near normal condition 14 days after disease attack.Cerebral infarction group was further subdivided into moderate,medium,heavy three groups.We found on day 7 of disease attack,comparison of the SOD and NOX2 levels between medium,heavy cerebral infarction groups and moderate,control groups showed statistical differences （P 〈 0.05 ）. Conclusion There exists dynamic peripheral blood NOX2 and SOD changes in patients with acute cerebral infarction,and it＇s correlated with the severity of cerebral infarc-tion,its concentration plays an important role in predicting disease changes and treatment.14 days.