云南蔗区首次发现由屈恩柄锈菌引起的甘蔗黄锈病

First Report of Orange Rust of Sugarcane Caused by Puccinia kuehnii in Yunnan Sugarcane Field

为明确在国家甘蔗体系云南示范点甘蔗新品种上发生的锈病病原菌种类。采用田间症状观察,结合病原菌形态特征观察及分子生物学方法对20份锈病样品进行病原菌鉴定。鉴定结果表明:来源于云南勐海‘海引1号’的4份甘蔗锈病样品属甘蔗黄锈病,病原菌为屈恩柄锈菌,其核苷酸序列与Gen Bank中登录的屈恩柄锈菌相应核苷酸序列(Gen Bank登录号:GU058021和GQ283004—GQ283009)同源性在99.9%以上,并在系统发育树中聚为一簇;来源于云南保山、临沧、勐海其他甘蔗新品种(系)的16份甘蔗锈病样品属甘蔗褐锈病,病原菌为黑顶柄锈菌,它们的核苷酸序列与Gen Bank中登录的黑顶柄锈菌相应核苷酸序列(Gen Bank登录号:EU164548,GU058001和JX036025)同源性在99.8%以上,在系统发育树中处于同一分枝。本研究首次在云南蔗区发现由屈恩柄锈菌引起的甘蔗黄锈病。

In order to identify the pathogens of sugarcane rust disease, which observed on sugarcane new cultivars in Yunnan demonstration site of the national sugarcane system in 2014. By using field symptom observation, urediniospore morphology and molecular identification method to determine the sugarcane rust pathogens of 20 rust samples. Identification results showed that 4 rust samples of new sugarcane variety ＇Haiyinl＇ collected from Menghai, Yunnan were confirmed to be sugarcane orange rust disease, the pathogen were identified to be Puccinia kuehnii Butler., their sequence shared more than 99.9% sequence identity with other reported isolates of P .kuehnii （GenBank Accession No.GU058021 and GQ283004-GQ283009）, and were clustered into one group in phylogenetic tree. Sixteen rust samples of new sugarcane varieties/clones collected from Baoshan, Lincang and Menghai in Yunnan were confirmed to be sugarcane brown rust disease, their pathogens were identified to be Puccinia me Syd. et P. Syd., they had sequence similarities of over 99.8% with other reported isolates of P. melanocephala （GenBank Accession No.EU164548, GU058001 and JX036025）, and in the same branch in phylogenetic tree. This is the first report of sugarcane orange rust disease caused by P.kuehnii in Yunnan sugarcane field.