贵州地方山羊CPT-1A基因在不同品种和组织中的表达差异

Analysis on expression differences of CPT-1A gene in different tissues of Guizhou native goat breeds

为揭示贵州地方山羊及南江黄羊CPT-1A mRNA水平的品种和组织差异,以黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊以及南江黄羊为研究对象,采用实时荧光定量方法对肝、肾、心、肺、背最长肌、半膜肌、皮下脂肪中CPT-1A基因的mRNA表达水平进行检测。结果表明,从器官组织分布模式来看,黔北麻羊和南江黄羊CPT-1A mRNA水平都以肾中最高(0.0019和0.0019),贵州黑山羊以肝中最高(0.0074),贵州白山羊以背最长肌中最高(0.0011),黔东南小香羊各组织中无差异;从品种差异来看,心和肾中CPT-1A mRNA水平以南江黄羊最高(0.0018和0.0019),肝和肺中以贵州黑山羊最高(0.0074和0.0027),皮下脂肪中以黔东南小香羊(0.0007)最高,背最长肌和半膜肌中各品种无差异。结果提示,贵州白山羊、贵州黑山羊、黔北麻羊以及南江黄羊CPT-1A基因的器官组织分布模式存在差异,肝、肾、心、肺和皮下脂肪中CPT-1A mRNA的表达存在品种间差异,此结果为研究该基因与贵州地方山羊脂肪沉积及肉质性状的关系提供了理论基础。

In order to reveal breed differences and tissue differences of CPT-1A mRNA levels in Guizhou native goats and Nanjiang yellow goats,Qiandongnan small-Xiang goats,Guizhou white goats,Guizhou black goats,Qianbei Ma goats and Nanjiang yellow goats were used as subjects,and expression levels of CPT-1A mRNA in livers,kidneys,hearts,lungs,longissimus muscle,amnion muscle and subcutaneous adipose were detected by real-time fluorescence quantitative RT-PCR. The results showed that the highest expression level（ 0. 0019 and 0. 0019） of CPT-1A mRNA in Qianbei Ma goat and Nanjiang yellow goat were observed in kidney. Similarly,the highest expression levels were observed in liver（ 0. 0074） of Guizhou black goat,and longissimus muscle（ 0. 0011） of Guizhou white goat as well.There were no significant difference among tissues in Qiandongnan small-Xiang goat. The highest expression levels of CPT-1A mRNA in heart（ 0. 0018） and kidney（ 0. 0019） were observed in Nanjiang yellow goat,which in liver（ 0. 0074） and lung（ 0. 0027） were observed in Guizhou black goat,and in subcutaneous adipose（ 0. 0007） wereobserved in Qiandongnan small-Xiang goat. There were no difference in longissimus muscle and amnion muscle among different breeds. The results suggested that tissue distribution models of CPT-1A mRNA in Guizhou white goat breed,Guizhou black goat breed,Qianbei Ma goat breed and Nanjiang yellow goat breed were different,and expression levels of CPT-1A gene in liver,kidney,heart,lung and subcutaneous adipose of different breeds were also different. This results provided the theoretical basis for study on the relationship between fat deposition or meat quality traits of Guizhou native goat breeds and CPT-1A gene.