番茄育种材料对黄化曲叶病毒抗性鉴定及抗性基因检测

Resistance evaluation of tomato breeding materials to Tomato yellow leaf curl virus and detection of Ty gene

以自育的26份番茄材料和抗番茄黄化曲叶病毒(TYLCV)品种杭杂301及感TYLCV品种阿乃兹为试材,采用田间自然发病、苗期病毒接种和PCR扩增及TaqⅠ酶切检测相结合,研究供试材料对TYLCV的抗性水平及基因型。结果表明,供试材料中,有高抗材料27份(包括抗病对照杭杂301)、感病1份(对照品种阿乃兹),且田间自然发病鉴定与苗期病毒接种鉴定的结果一致。分子鉴定结果表明,除了感病对照品种阿乃兹和24号材料外,其他26份番茄材料均能检测到显性Ty基因,其中21份材料含有Ty纯合基因,5份材料含有Ty杂合基因,未发现材料中含有Ty-4和Ty-5基因,分子检测结果与田间人工接种和苗期鉴定的结果吻合率达96.4%。含显性Ty基因的材料,均表现出较好的抗TYLCV特性,但含不同抗性基因的材料,抗性表现不同,而携带2个或多个抗病基因的材料,其抗性水平更高更稳定。采用分子检测与抗性鉴定相结合,能将2个或多个抗性基因聚合到1个材料上,可培育出抗性更高、更广、更持久的优良番茄新品种。

Twenty-six autogeny lines were selected to assess resistant levels to TYLCV and genotypes by natural incidence in field,seedling artificial inoculation,PCR amplification and TaqⅠ restriction enzyme. Anaizi and Hangza301 were used as susceptible control and resistant control,respectively. The results showed that 27 tested materials all were high resistant to TYLCV,1 tested material was susceptible to TYLCV,and the results of identification of natural incidence was consistent with artificial inoculation. According to molecular detection,26 tested autogeny materials contains dominant Ty gene except Anaizi and No. 24 material,21 of which contain homozygous gene,5 of which contain heterozygous gene. Ty-4 and Ty-5 gene were not detected among tested materials. The coincidence rate reached above 96. 4% among molecular detection,artificial inoculation and natural incidence in field. It was indicated that materials containing dominant Ty showed superior resistant symptoms,while materials containing different resistant genes had different resistant symptoms. Materials carrying two or more resistant genes had higher and morestable resistant level. So combination of molecular detection and field identification could get a material containing two or more resistant genes which might have higher,broader and more stable resistance.