正交试验优选蓝莓抗疲劳口服液提取工艺

Optimization of Extraction Technology of Blueberry Anti-fatigue Oral Liquid by Orthogonal Test

为优选蓝莓抗疲劳口服液的提取工艺,以多糖、总黄酮含量为考察指标,以煎煮时间、煎煮次数、蓝莓白术当归的比例、料液比为考察因素,采用正交实验优选蓝莓抗疲劳口服液的提取方法.优选提取条件为:以多糖为考察指标,15倍量水煎煮3次,每次2 h,配伍比,蓝莓∶白术当归(2∶1);以总黄酮为考察指标,20倍量水煎煮2次,每次3 h,配伍比,蓝莓∶白术当归(2∶1).实验结果表明,该提取方法简单,易于操作.

This paper takes the contents of polysaccharides and total flavonoids as research indices, and takes decoction time, decoction times, compatibility proportion of blueberry to arractylodes and angelica, and solid-liquid ratio as research factors. By orthogonal test,the extraction technology of blueberry anti-fatigue oral liquid is optimized. The experimental results show that the optimal extraction conditions are as follows： polysaccharides as index： decocted three times with 15 times the amount of water, each for 2 hours;total flavonoids as index： decocted two times with 20 times the amount of water, each for 3 hours. Their compatibility proportions are both 2 ： 1. We conclude that this optimal technology is simple and easy for operation, which can provide a basis for practical application in the future.