摘要
利用昆虫遗传转化技术对害虫进行遗传控制是害虫防治研究的新方向,该技术具有物种特异、防效高且对环境友好的特点。启动子是基因表达调控的重要元件,选择合适的启动子是外源基因高效、准确表达的关键,对获得高效、稳定的遗传修饰昆虫品系至关重要。本文简要介绍了昆虫基因启动子的结构特征,重点描述了昆虫种群遗传防治中组成型启动子、性别和组织特异型启动子、特定发育时期启动子和诱导型启动子的研究和应用概况,并对这几类启动子在害虫遗传控制中的应用前景进行了展望。
Genetic control using germ-line transformation is the new trend in the research of insect pest control. It is a species-spe- cific, highly efficient and environmental friendly pest control strategy. Gene promoter, one of the key component for gene expression regulation, is requisite for the construction of high-efficient and stable genetic modified strains for insect population genetic control. In this review, the basic structural characteristics of insect gene promoters are briefly described. The progress and application of dif- ferent type promoters such as constitutive promoter, sex- and tissue-specific promoter, age-specific promoter and inducible promoter, are also introduced. Finally, the future prospect of different types of promoters used in insect population genetic control is disccused.
出处
《生物安全学报》
2015年第2期115-125,共11页
Journal of biosafety
基金
环保公益性行业科研专项(201409061)
农业部2014年农作物病虫鼠害疫情监测与防治(外来入侵生物防治)项目
人力资源社会保障部2014年度留学人员科技活动择优资助项目
关键词
遗传防治
昆虫不育技术
遗传转化
组成型启动子
特异性启动子
诱导型启动子
insect population genetic control
sterile insect technique
genetic transformation
constitutive promoter
specific pro-moter
inducible promoter
