小檗碱对糖尿病肾病大鼠肾脏损伤的保护作用

Berberine Ameliorates Renal injury in Diabetic Nephropathy Rats

目的:探讨小檗碱对糖尿病肾病(diabetic nephrpathy,DN)大鼠肾脏损伤的保护作用。方法:健康成年雄性SD大鼠40只,随机分为正常对照组、模型组(DN组)和小檗碱组。DN组及小檗碱组大鼠行左侧肾摘除术,术后2周腹腔一次性注射链脲佐菌素(streptozotocin,STZ)60mg/kg诱导DN大鼠模型,72h后测非空腹血糖>16.8mmol/L为造模成功;小檗碱组大鼠给予小檗碱0.1g/(kg·d)灌胃。定时检测各组大鼠血糖、尿微量白蛋白和肾小球滤过率的变化。结果:与DN组大鼠相比,小檗碱组大鼠血糖和24h尿微量白蛋白明显降低,肾小球滤过率增加(P<0.05),肾功能明显改善。结论:小檗碱可明显降低DN大鼠血糖,改善DN大鼠肾功能紊乱,对DN大鼠肾脏具有较强的保护作用。

Objective：To investigate the protective effects of Berberine on renal injury in Diabetic Nephropathy（DN）Rats.Methods：40healthy male SD rats were randomly divided into control group,DN group,Berberine group.Diabetic model was established by administration of Streptozotocin（STZ）in a single dose of 60mg/kg intraperitoneally after the left kidney was removed 2weeks and the wound was healed.The model was considered successful when the level of blood glucose was above 16.8mmol/L at 72 hafter operation.The rats in the Berberine group was treated with Berberine in a dose of 0.1g/（kg·d）by gavage,once a day.The change of blood glucose,urine microalbumin,glomerular filtration rate（GFR）was detected.Results：Compared with DN group,the blood glucose,24 hmicroalbumin in Berberine group was obviously decreased,while GFR was increased（P〈0.05）.Conclusion：Berberine can decrease blood glucose,ameliorate renal dysfunction,suggesting that it has obvious renal protective effect on diabetic nephropathy rats.