白藜芦醇对大鼠肝脏缺血再灌注损伤的保护作用及机制研究

Protective Effect and Mechanism of Resveratrol on Hepatic Ischemia Reperfusion Injury in Rats

目的:研究白藜芦醇在肝脏缺血再灌注损伤(CHIRI)中的保护作用及其相关机制。方法:24只雄性大鼠随机分为假手术对照(SC)组、缺血再灌注损伤(I/R)组、白藜芦醇后处理(Res)组,每组8只。建立大鼠在体肝脏缺血再灌注模型,于再灌注6h采集血液和肝组织标本。观察肝脏病理学变化,检测血清中肝脏酶学指标ALT、AST、AKP和炎症介质指标TNF-α、IL-6、IL-10、TGF-β的变化。结果:白藜芦醇后处理可以减轻肝脏病理损伤,显著降低肝脏酶学指标,同时促炎介质TNF-α和IL-6明显下降,抑炎介质IL-10和TGF-β含量明显升高,与I/R组比较,各指标变化均有显著性差异(P<0.01)。结论:白藜芦醇对大鼠HIRI有明显的保护作用,其机制可能是通过抑制TNF-α和IL-6的释放,促进IL-10和TGF-β的分泌,从而抑制炎症反应实现。

Objective：To investigate the protective effect of resveratrol on hepatic ischemia-reperfusion injury in rats and to study the related mechanism.Methods：24male SD rats were randomly divided into three equal groups：sham-operated control group（SC）,ischemia/reperfusion injury group（I/R）,post-disposal treatment of resveratrol group（Res）.70% hepatic ischemia/reperfusion model was established.The blood sample and liver tissue samples were collected 6hours after reperfusion.The pathological changes of the liver were observed.Serum ALT,AST and AKP activity were detected as indicators of liver function damage and the content of TNF-α,IL-6,IL-10 and TGF-βin serum were detected by ELISA.Results：Resveratrol treatment can improve the pathological injury of the liver.It could also effectively serum ALT,AST and AKP levels.Compared with the I/R group,TNF-αand IL-6levels dramatic declinedwhile IL-10 and TGF-βlevels obviously increased in the resveratrol treated groups（P〈0.01）.Conclusion：Resveratrol can improve the rat liver function and pathological damage,though the mechanisms of reducing the inflammatory responseby restraining the release of proinflammatory cytokine including TNF-αand IL-6and promoting the release of antiinflammatory cytokine including IL-10 and TGF-β.