Th17细胞在严重全身性感染患者外周血中的表达及临床意义

Expression of Th17 cells in peripheral blood of patients with severe systemic infection and its clinical significance

目的:检测严重全身性感染患者外周血Th17/CD4+T细胞的比例及单个核细胞(PBMC)中RORγtmRNA和IL-17 mRNA的表达水平,探讨Th17细胞在严重全身性感染患者外周血中的临床意义。方法:采用流式细胞分析法检测20例严重全身性感染患者、20例感染性休克患者及20例健康体检者外周血Th17/CD4+T细胞比例,RT-PCR法检测PBMC中RORγt mRNA及IL-17 mRNA的水平。结果:严重全身性感染组及感染性休克组外周血Th17/CD4+T细胞比例、RORγtmRNA及IL-17 mRNA水平均高于健康对照组,且严重全身性感染组高于感染性休克组(均P<0.05)。结论:严重全身性感染及感染性休克患者外周血Th17/CD4+细胞比例增加,RORγt及IL-17蛋白表达水平升高,Th17细胞参与了炎症的发生、发展。

Objective： To detect the Th17 / CD4＋T cells ratio in peripheral blood and the expression of RORγt mRNA and cytokine IL-17 mRNA in peripheral blood mononuclear cells（ PBMC） in patients with severe sepsis,and to explore its clinical significance. Methods： Flow Cytometry（ FCM） was used to detect the peripheral blood ratio of Th17 / CD4^＋T cells in20 cases of severe systemic infection,20 cases of septic shock patients and 20 healthy subjects,reverse transcription PCR（ RT-PCR） was used to detect the expressions of RORγt and cytokine IL-17 mRNA in PBMC of all groups. Results： The expressions of the peripheral blood ratio of Th17 / CD4^＋T cells and RORγt mRNA and cytokine IL-17 mRNA in PBMC of patients with severe systemic infection and septic shock were higher than those of healthy control group. The expressions of the peripheral blood ratio of Th17 / CD4^ ＋ T cells and expressions of RORγt mRNA and cytokine IL-17 mRNA in PBMC in severe systemic infection were higher than those of septic shock group（ P〈0. 05）. Conclusions： The expression of the peripheral blood ratio of Th17 / CD4^＋T cells in patients of severe systemic infection and septic shock,and its expressions of RORγt and cytokine IL-17 mRNA in PBMC are increased,all these suggest that Th17 cell may involve in occurrence and development of inflammation.