CIA小鼠血清中IL-27、IL-10、IL-17的动态分析

Dynamic changes of IL-27,IL-10,IL-17 in serum of collagen-induced arthritis in mice

目的:观察鸡Ⅱ型胶原诱导型关节炎(CIA)小鼠血清中IL-27、IL-17、IL-10的动态变化。方法:54只DBA1/J小鼠随机分为对照组(每组6只)和模型组(每组12只),分别在CIA小鼠病程进展的早、中、晚期无菌摘眼球取血,常规分离血清,应用流式细胞术检测各组小鼠在疾病病程的早、中、晚期血清中细胞因子IL-27、IL-17、IL-10的动态变化情况。结果:模型组IL-27在疾病早期、中期显著下降(P<0.05;P<0.01),疾病晚期与对照组比较差异无统计学意义(P>0.05);模型组IL-17在疾病的早期及晚期与对照组比较无明显区别(P>0.05),而在病程中期显著高于对照组(P<0.05)。IL-10在CIA小鼠的病程进展中,模型组与对照组相比差异无明显区别(P>0.05)。结论:细胞因子IL-27和IL-17、IL-10可能参与CIA发病,可能与胶原诱导型关节炎的炎症进展有一定关系。

Objective： To observe chicken type Ⅱ collagen-induced arthritis（ CIA） in serum of mice with the dynamic changes of IL-27,IL-17,IL-10. Methods： 54 DBA1 / J mice were randomly divided into control group（ n = 6） and model group（ n = 12）,according to the progress of CIA mouse course early,middle and late（ 7,14,and 35 days after booster immunization）,taking the eyeball for blood and separating the serum under sterile condition. The dynamic levels of cytokines IL-27,IL-17,IL-10 were detected by flow cytometry. Results： The level of IL-27 in the model group was significantly declined in the the progress of CIA mouse course early and middle（ P〈0. 05,P〈0. 01） compared with the control group,but there was no significant difference between late disease and control group（ P〉0. 05）; the level of IL-17 in the disease early and late was no significant difference（ P〉0. 05） compared with the control group,while in the mid course significantly higher than control group（ P〈0. 05） during the course of disease. The level of IL-10 showed no difference from the experiment beginning（ P〉0. 05）. Conclusion： IL-27,IL-17,IL-10 paticipate in the pathogenesis of CIA and their alterations at different stages of the disease have a relation to the development of arthritis.