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microRNA-204在鼻咽癌中的表达及生物学意义研究 被引量:5

Expression and biological significance of microRNA-204 in nasopharyngeal carcinoma
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摘要 目的:研究微小RNA 204(microRNA-204,miR-204)在鼻咽癌(Nasopharyngeal carcinoma,NPC)中的表达情况及生物学意义。方法:收集2012年3月至2014年3月间于我院耳鼻喉头颈外科行组织病理活检的NPC组织及对应癌旁鼻黏膜组织共43例,运用q RT-PCR技术检测miR-204在NPC组织及癌旁组织中的表达水平,统计分析miR-204表达水平与患者临床病理资料间的相关性;免疫组化检测鼻咽癌组织中miR-204下游潜在靶点Bcl-2及SIRT1蛋白的表达水平,统计分析Bcl-2及SIRT1蛋白与miR-204表达间的相关性;采用人工合成的miR-204模拟物(miR-204 mimics)转染人鼻咽癌CNE-2细胞,分别采用q RT-PCR及Western blot检测转染后Bcl-2及SIRT1 mRNA和蛋白的表达变化。结果:miR-204在NPC组织中表达水平显著低于对应癌旁鼻黏膜组织(P<0.05);miR-204低表达与肿瘤淋巴结转移(P<0.05)及高TNM分期(Ⅲ+Ⅳ期,P<0.05)显著相关;在鼻咽癌组织中miR-204与其下游潜在靶点Bcl-2及SIRT1的表达呈显著负相关关系(P<0.05),miR-204转染人鼻咽癌CNE-2细胞后可显著降低细胞内Bcl-2及SIRT1 mRNA和蛋白的表达水平(P<0.05)。结论:miR-204在鼻咽癌组织中表达下调并与肿瘤恶性临床病理特征有关,miR-204可能通过下调Bcl-2及SIRT1的表达来抑制鼻咽癌的发生、发展过程。 Objective: To investigate the expression and biological significance of Micro RNA-204 in nasopharyngeal carcinoma( NPC). Methods: q RT-PCR was applied to detect the relative expression of miR-204 in 43 paired nasopharyngeal carcinoma in comparison to the normal nasal mucosa tissues. Pearson chi-square test was used to analyze the relationship between the miR-204 expression and clinical features. The expressions of Bcl-2 and SIRT1 were measured by immunohistochemistry( IHC),Spearman correlation analysis was used to analyze the relationship between miR-204 and Bcl-2,as well as SIRT1. We then transfected the miR-204 mimics into CNE-2 cells,then the Western blot was used to detect the expression of Bcl-2 and SIRT1,which were considered as the potential targets of miR-204. Results: The relative expressions of miR-204 was significantly downregulated in NPC tissues compared to those of the matched normal tumor-adjacent tissues( P〈0. 05). Low expression of miR-204 was significantly associated with lymphatic metastasis( P〈0. 05) and advanced TNM stage( Ⅲ + Ⅳ,P〈0. 05). The expressions of Bcl-2 and Sirt1 in lower miR-204 level group were both higher than in higher miR-204 level group( P〈0. 05). Both the m RNA and protin expression in CNE-2 cells were down-regulated after transfection. Conclusion: Low expression of miR-204 is related to the malignant clinicopathological features in NPC tissues,and miR-204 may through down-regulate Bcl-2 and SIRT1 to suppress NPC genesis and development.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2015年第6期822-826,共5页 Chinese Journal of Immunology
关键词 MicroRNA-204 鼻咽癌 BCL-2 SIRT1 MircoRNA-204 Nasopharyngeal carcinoma Bcl-2 SIRT1
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  • 1王盼盼,季明芳,吴标华.鼻咽癌高发区人群患鼻咽癌风险率的动态观察[J].中华肿瘤防治杂志,2014,21(15):1144-1147. 被引量:16
  • 2Lee FK, Yip CW, Cheung FC, et al. Dosimetric difference amongst 3 techniques : TomoTherapy, sliding-window intensity-modulated ra- diotherapy( IMRT), and RapidArc radiotherapy in the treatment of late-stage nasopharyngeal carcinoma ( NPC ) [ J ]. Med Dosim, 2014,39( 1 ) :44.49.
  • 3Dvinge H, Git A, Grf S, et al. The shaping and functional consequences of the mieroRNA landscape in breast cancer [ J ]. Nature ,2013,497 (7449) :378-382.
  • 4Guang-Da Yang, Tie-Jun Huang, Li-Xia Peng, et al. Epstein-Barr VirusEncoded LMP1 Upregulates MicroRNA-21 to Promote the Resistance of Nasopharyngeal Carcinoma Ceils to Cisplatin-Induced Apoptosis by Suppressing PDCD4 and Fas-L[ J]. PLoS One,2013, 8(10) :e78355.
  • 5Chen X, Wang J, Cheng L, et al. rniR-18a dowm'egulates DICER1 and promotes proliferation and metastasis of nasopharyngeal carcinoma [ J ]. Int J Clin Exp Met],2014,7 (4) :847-855.
  • 6Ma L, Deng X, Wu M, et 02. Down-regulation of miRNA-204 by LMP-1 enhances CDCA2 activity and facilitates invasion of EBV- associated nasopharyngeal carcinoma cells [ J ]. FEBS lett, 2014, 588(9) :1562-1570.
  • 7Ryan J, Tivnan A, Fay J, et al. MieroRNA-204 increases sensitivity of neuroblastoma cells to cisplatin and is associated with a favourable clinical outcome [ J ]. Br J Cancer, 2012, 107 ( 6 ) : 967 -976.
  • 8Li W, Jin X, Zhang Q, et al. Decreased expression of miR-204 is associated with poor prognosis in patients with breast cancer[ J ]. Int J Clin Exp Pathol,2014,7(6) :3287-3292.
  • 9Li C,Yang W,Zhang J,et al. SREBP-1 has a prognostic role and contributes to invasion and metastasis in human hepatocellular carcinoma[ J]. Int J Mol Sei ,2014,15 (5) :7124-7138.
  • 10何跃平,罗晶晶,刘钊,安立,李祥东,张艳.靶向livin的siRNA对鼻咽癌细胞增殖和凋亡的影响[J].中国免疫学杂志,2014,30(8):1028-1031. 被引量:9

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