奶牛乳腺炎停乳链球菌的分离鉴定及耐药性分析

Isolation,Identification and Drug Resistance Analysis of Streptococcus Dysgalactiae from Bovine Mastitis Milk Sample

对甘肃省部分地区奶牛场的323份乳腺炎乳样,采用6％～8％绵羊鲜血琼脂平板培养基、选择性培养基（BETA-SSA琼脂培养基）、普通营养琼脂培养基,结合革兰染色、镜检,对可疑菌落分离纯化后进行生化试验,确定分离出停乳链球菌;同时选用临床常用的10种抗生素对分离株进行药敏试验,旨在研究停乳链球菌的分离及分离株的耐药性分析,为临床合理用药提供科学依据.结果表明：216株分离株对青霉素、四环素、红霉素表现出明显的耐药性,其耐药率分别为71.30％、59.26％、52.78％,而对左氧氟沙星、万古霉素、庆大霉素、甲砜霉素高度敏感,其敏感率分别为76.39％、73.61％、65.74％、64.35％.

In order to provide a scientific basis for rational clinical use of drug,streptococcus dysgalactiae from 323 mastitis dairy cows in some region in Gansu were used in this study.Streptococcus dysgalactiae was cultured using 6-8% BETA-SSA agar medium,common nutrient agar plate first,then separated and purified by Gram＇s stain and microscope detection technology.Ten frequently-used antibiotics were used in drug sensitive test.The result indicated that 216 strains of Streptococcus dysgalactiae were highly drugfast to penicillin,tetracycline and erythrocin.The drug resistance rate were 71.30%,59.26%,52.78%,respectively.While the susceptible rate of levofloxacin,vancomycin,gentamicin and thiamphenicol were 76.39%,73.61%,65.74% and 64.35%,respectively.