瓦布贝母乙醚提取物GC-MS分析及其抗氧化活性评价

GC-MS Analysis of Ether Extract from Fritillaria wabuensis and Its Antioxidant Activity

【目的】分析瓦布贝母鳞茎乙醚提取物中化学成分及其抗氧化活性。【方法】瓦布贝母鳞茎干、鲜样分别采用索氏提取法和超声提取法以乙醚为溶剂进行提取,并结合GC-MS分析其化学成分,用面积归一法研究其相对含量,再用DPPH自由基和ABTS+自由基清除法评价其抗氧化活性。【结果】超声提取法提取效率较好,从超声鲜样(CX),索氏鲜样(SX),超声干样(CG)和索氏干样(SG)的提取物中分别鉴定出69,58,73和57种成分,分别占其总成分百分比为62.16%,53.70%,65.18%和58.76%,占其总含量百分比为80.01%,68.91%,68.62%和74.89%。所得成分中十六烷酸、亚油酸乙酯、亚油酸和邻苯二甲酸单(2-乙基己基)酯为主要成分。此外,所有样品都表现出一定的自由基清除活性,CX、SX、CG和SG的DPPH自由基清除率的IC50分别为30.66、29.77、15.99和21.11mg/mL,ABTS+自由基清除率的IC50分别为35.72、21.08、23.40和15.45mg/mL。【结论】不同提取方法和不同样品状态对其主要成分的种类含量影响较小;4种测定样主要成分基本一致,但低含量的成分差别较大;并且瓦布贝母乙醚溶解成分有一定的抗氧化活性;所用GC-MS分析方法简便、快速、灵敏度高,结果可进一步促进对瓦布贝母药效物质基础的认识。

[Objective]The aim of the study was to study the chemical composition of the ether ex-tract from Fritillaria wabuensis and its antioxidant activity.[Method]GC-MS was used to detect the ether extract from the dry and fresh samples of F .wabuensis by soxhlet extraction method （SEM）and ultrasonication extraction （UE）,respectively.The relative contents of chemical com-position from F .wabuensis were determinated by area normalization.The antioxidant capacity was evaluated by DPPH and ABTS＋ radical scavenging assay.[Results]There was higher extraction efficiency by UE than SEM.69 and 58 kinds of components from the fresh samples by SEM and UE were determined but 73 and 57 kinds of components from dry samples by SEM and UE were determined, respectively. Moreover, the percentages of those components were 62.16%, 53.70%,65.18% and 58.76% in the total number of components as well as 80.01%,68.91%, 68.62% and 74.89% in the total content,respectively.The main components were n-hexadecano-ic acid,linoleic acid ethyl ester,（Z,Z）-9,12-octadecadienoic acid and 1,2-benzenedicarboxylic＆amp;nbsp;acid,mono （2-ethylhexyl）ester in all samples.The ether extracts from fresh and dry samples by SEM and UE all showed antioxidant activity （IC50 values were 30.66,29.77,15.99 and 21.11 mg/mL with DPPH;35.72,21.08,23.40 and 15.45 mg/mL with ABTS＋ ,respectively）.[Con-clusion]The different extract methods and treatments had a relatively little effect on the main components.However,there was obvious difference in the low content ingredients in different samples.Furthermore,the ether extracts from F .wabuensis had certain antioxidant activity.GC-MS is a simple,sensitive and rapid method for determination of the chemical composition.It is helpful for our understanding of F .wabuensis .