高邮鸭群体MSTN、MyoD1和MyoG基因外显子中SNP位点的分析

Analysis of single nucleotide polymorphisms(SNPs) in exons of MSTN,MyoD1 and MyoG genes of Gaoyou duck

MSTN、MyoD1和MyoG基因与禽类骨骼肌的生长发育密切相关。本试验以高邮鸭为素材,对MSTN、MyoD1和MyoG3个基因的外显子单核苷酸多态性(SNP)位点进行了鉴定,并对这些位点对蛋白质的影响及群体遗传信息进行了分析。结果显示,MSTN、MyoD1和MyoG3个基因的外显子分别有9个、4个和7个SNP位点,其中具有中度多态的位点分别为8个、1个和5个,有义突变各1个,这些有义突变均改变了相应蛋白质的理化性质以及二级结构。

There are strong relations between MSTN, MyoD1, MyoG genes and development of skeletal muscle. The single nucleotide polymorphisms （ SNPs） in the exons of MSTN, MyoD1 and MyoG genes of Gaoyou duck were detected, and their influences on proteins and population genetics information were studied. There were nine, four SNPs and seven SNPs in the exons of MSTN, MyoD1 and MyoG genes, respectively, among which, eight, one and five SNPs were moderate polymorphic. One sense mutation detected in each gene changed the protein physico-chemical characteristics and second structure.