莲蓬壳提取物不同极性部位的生物活性

Bioactivities of different fractions of lotus receptacle

为了确定莲蓬壳的生物活性物质,采用有机溶剂萃取法将莲蓬壳提取物分为正己烷相、二氯甲烷相、乙酸乙酯相、正丁醇相和水相5个不同极性部位,测定不同极性部位提取物中总黄酮及多酚含量,分析其还原能力及对过氧化氢、超氧阴离子自由基和DPPH自由基的清除能力,比较莲蓬壳提取物不同极性部位提取物的抗氧化作用。同时还考察了莲蓬壳不同极性部位提取物对乳腺癌细胞(MCF-7)的抑制作用。结果显示:莲蓬壳提取物的不同极性部位提取物均有抗氧化活性作用,其中乙酸乙酯部位提取物和正丁醇部位提取物抗氧化能力较强于其他萃取相部位提取物;正己烷相提取物和二氯甲烷相提取物可显著抑制MCF-7细胞的增殖。

Five fractions of lotus receptacle, hexane, dichloromethane（ CH2 Cl2 ） , ethyl acetate（ EtOAc） , n-butanol （ N-BuOH） and water （ H2 O） , extracted with ethanolic were determined for their total phenolic and flavonoid contents and antioxidative activities. Total phenolic and flavonoid contents exhibited the highest contents in EtOAC fraction, followed by N-BuOH fraction and water fraction in order. Antioxidations of all five fractions were shown by the strong scavenging abili-ties to hydrogen peroxide, superoxide anion and 1-dipheny1-2-picrylhydrazy1. The two fractions, EtOAC and N-BuOH, outperformed other three in antioxidation, while other two fractions, CH2 Cl2 and hexane, were superior in inhibiting the proliferation of human breast cancer cell（MCF-7）.