小蓟化学成分的分离与鉴定

Isolation and indentification of chemical constituents from Cirsium setosum(Willd.) MB.

目的研究小蓟的化学成分。方法采用硅胶、凝胶、ODS柱色谱以及HPLC制备法对小蓟的乙醇提取物进行分离纯化,利用理化性质和波谱学手段鉴定其结构。结果从小蓟乙醇提取物中分离得到14个化合物,经鉴定分别为邻二苯酚（1,2-benzenediol,1）、反式对香豆酸葵酯（decyl transp-coumarate,2）、原儿茶醛（protocatechualdehye,3）、对羟基苯甲酸（p-hydroxybenzoic acid,4）、香草酸（vanillic acid,5）、刺槐素（ermanine,6）、岳桦素（acacetin,7）、马齿苋酰胺（oleracein E,8）、芹菜素（apigenin,9）、木犀草素（luteolin,10）、槲皮素（quercetin,11）、异山柰素-7-O-β-D-葡萄吡喃糖苷（isokaempferide-7-O-β-D-glucopyranoside,12）、高车前苷（homoplantaginin,13）和蒙花苷（linarin,14）。结论化合物1-4、7、8和13为首次从小蓟中分离得到。

Objective To study the chemical constituents of Cirsium setosum（ Willd.） MB. Methods Column chromatography of silica gel,Sephadex LH-20,ODS and preparation HPLC were used to isolate the chemical constituents from alcohol extract of C. setosum（ Willd.） MB. Their structures were confirmed on their physic-chemical property and NMR spectroscopic analyses. Results Fourteen compounds were purified and their structures were identified as 1,2-benzenediol（ 1）,decyl trans-p-coumarate（ 2）,protocatechualdehye（ 3）,p-hydroxybenzoic acid（ 4）,vanillicacid（ 5）,ermanine（ 6）,acacetin（ 7）,oleracein E（ 8）,apigenin（ 9）,luteolin（ 10）,quercetin（ 11）,isokaempferide-7-O-β-D-glucopyranoside（ 12）,homoplantaginin（ 13）,linarin（ 14）.Conclusions Compounds 1-4,7,8 and 13 are isolated from Herba cirsii for the first time.