Y盒结合蛋白1表达上调与宫颈癌上皮-间质转化之间的相关性研究

Correlation of YB-1 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma

目的：探讨Y盒结合蛋白1（YB-1）在宫颈癌中表达的临床病理意义及与上皮-间质转化的相关性。方法选取202例标本，包括50例正常宫颈组织，100例宫颈上皮内瘤变（CIN）及52例鳞状细胞癌（SCC），进行 YB-1和上皮型-钙黏蛋白（E-cadherin）的免疫组织化学分析。结果 E-cadherin 在正常宫颈组及膜表达率为100%，CINⅡ～Ⅲ组为64%，SCC 组仅为3.85%，正常宫颈/ CINⅠ组比 CINⅡ～Ⅲ、正常/ CINⅠ比 SCC、CINⅡ～Ⅲ比 SCC 分别为χ^2=40.909；χ^2=119.088；χ^2=25.274；均 P 〈0.05。E-cadherin阴性及畸形表达率在转移 SCC 组和非转移 SCC 组中分别为100.0%（20/20）和68.75%（22/32），连续性校正性χ^2=5.857，P =0.016。YB-1在 SCC 病例胞质中表达为94.23%（49/52），CINⅡ～Ⅲ组表达率为10.00%（5/50），正常宫颈组和 CINⅠ组表达率为0（0/100），正常宫颈/ CINⅠ组比 CINⅡ～Ⅲ、正常/ CINⅠ比 SCC、CINⅡ～Ⅲ比 SCC 分别为χ^2=72.591；χ^2=139.059；χ^2=5.857；均P 〈0.05。在宫颈癌的进展过程中，YB-1在早期 SCC 胞质表达率为60.71%（17/28），晚期 SCC 为91.67%（22/24），两组相比差异有统计学意义（χ^2=6.603，P =0.01）。转移组和非转移组中分别为100.00%（20/20）和59.38%（19/32），两组相比差异有统计学意义（χ^2=10.833，P =0.001）。结论 YB-1过表达和宫颈上皮的恶性转化、分期进展及转移能力相关，YB-1过表达也与 E-cadherin 低表达相关，可能是预测CIN 癌变和宫颈癌侵袭转移的标志物。

Objective To investigate the clinicopathologic significance of Y-box binding protein-1 （YB-1）expression in cervical cancer and its correlation with epithelial-mesenchymal transition. Methods A series of 202 samples,including 50 cases of normal cervical tissues,100 cases of cervical intraepithelial neo-plasia（CIN）and 52 cases of squamous cell carcinoma（SCC）,were examined YB-1 and E-cadherin by immu-nohistochemical staining. Results The E-cadherin cell membrane immunoreactivity for normal/ CINⅠ,CINⅡ-Ⅲ,and SCC tissues were 100% ,64% and 3. 85% ,respectively（ χ^2 = 40. 909;χ^2 = 119. 088;χ^2 =25. 274;P 〈 0. 05）. The negative and aberrant expression of E-cadherin was higher in metastatic SCC （100. 0% ,20 / 20）than that in non-metastatic SCC（68. 75% ,22 / 32）（χ^2 = 5. 857,P = 0. 016）. 94. 23%（49 / 52）cases of SCC exhibited strong YB-1 cytoplasmic immunoreactivity. The positive rates in normal/ CINⅠand CINⅡ-Ⅲ were 0（0 / 100）and 10. 00%（5 / 50）（χ^2 = 72. 591;χ^2 = 139. 059;χ^2 = 5. 857;P 〈 0. 05）. The cytoplasmic expression of YB-1 was higher in metastatic SCC（100. 0% ,20 / 20）than that in non-metastatic SCC（59. 38% ,19 / 32）（χ^2 = 10. 833,P = 0. 001）. The rates were 60. 71%（17 / 28）and 91. 67%（22 /24）in early stage SCC and late stage SCC（χ^2 = 6. 603,P = 0. 01）. Conclusion YB-1 over-expression is as-sociated with the malignant transformation of cervical epithelium,stage progression and metastasis of cervical cancer. The up-regulation of YB-1 is also associated with the down-regulation of E-cadherin,and it may predict the malignant transformation of CIN and distal metastasis of cervical cancer.