发酵灵芝胞外多糖硫酸化修饰

Sulfated Modification of Extracellular Polysaccharide from Submerged Fermentation of Ganoderma lucidum

发酵灵芝胞外多糖表现出微弱的抗肿瘤活性,硫酸化是改善多糖抑癌活性的重要途径,而多糖的硫酸化取代度与其生物活性密切相关。为了了解硫酸化取代度与硫酸化条件之间的关系,以获取不同取代度的产品,对发酵灵芝胞外多糖的硫酸化试剂以及硫酸化反应条件与取代度之间的关系进行了较深入研究。实验结果表明,氨基磺酸比氯磺酸的硫酸化过程更加缓和,尿素的加入也有利于缓和反应条件,提高取代度。

The extracellular polysaccharides from submerged fermentation of Ganoderma lucidum exhibit low anticancer activity. Sulfation of polysaccharides is an important approach to improve anticancer activity of polysaccharides. In addition, the degree of sulfated substitution （DS） is closely related to its biological activities. In order to understand the relationship between DS and sulfated conditions and obtain the sulfated derivatives with different DS, the sulfuric acid esterification reagent and the relationship between the sulfated reaction conditions and DS were further investigated. The results showed that aminosulfonic acid offered milder reaction conditions than that of chlorosulfonic acid. On the other hand, the addition of urea also gave mild reaction conditions and improved DS.