丹参、黄芪注射液对自体脂肪移植存活率的影响

The Effects of Salvia miltiorrhiza and Astragalus propinquus Injection on the Survival of Autologous Adipose Tissue Transplantation

目的观察中药丹参、黄芪注射液对自体脂肪移植成活率的影响,为后续实验及临床应用提供依据。方法建立新西兰白兔自体脂肪移植动物模型,实验组分别予以腹腔注射丹参、黄芪注射液,于不同时间点采血、取材,测定血清VEGF表达水平和脂肪存活率,HE染色观察组织细胞形态,血小板-内皮细胞黏附分子CD31、周脂素免疫组化染色观察毛细血管密度及细胞活性。结果自体脂肪移植术后第12周,实验组脂肪存活率显著高于对照组;各时间点实验组血清VEGF表达水平均显著高于对照组;HE、CD31、周脂素染色示,实验组在细胞形态、新生毛细血管密度及细胞活性方面均明显优于对照组。结论配合应用丹参、黄芪注射液,能够显著提高新西兰白兔自体脂肪移植的存活率。

Objective To explore the effects of Salvia mihiorrhiza and Astragalus propinquus injection on the survival of transplanted grafts, so as to provide evidence for further research and clinical application. Methods Autologous adipose tissue transplantation model was established using New Zealand white rabbit. The experimental groups were given S. miltiorrhiza and A. propinquus injection intraperitoneally. The expression levels of VEGF in serum and survival rates were measured and calculated at different time points. Tissue morphology was observed by HE staining. The expression of platelet endothelial cell adhesion molecule-1 （CD31） and perilipin were observed by immune-histochemistry to evaluate the neocapillary growth and cyto-activity of transplanted adipose tissue. Results Twelve weeks after transplantation, the survival rates in experimental groups were higher than the control group. The expression levels of VEGF in serum in experimental groups were significantly higher than those in the control group at each time point. HE and immuno-histochemical staining demonstrated better morphology, denser neo-capillaries and higher cyto-activity in experimental groups, compared with the control group. Conclusion By applying S. mihiorrhiza and A. propinquus injection, the survival of autologous transplanted adipose tissue can be significantly enhanced in New Zealand rabbits.