基于超高效液相色谱指纹图谱的正交试验优选“二神丸”药对醇提工艺考察

Optimization of ethanol extraction process of ‘Ershen pills 'drug pair by orthogonal test based on UPLC fingerprints

目的:采用超高效液相色谱(UPLC)指纹图谱结合正交试验优选"二神丸"药对最佳醇提工艺。方法:选择乙醇浓度、乙醇用量、提取时间、提取次数4个因素,采用L9(34)进行正交试验,以UPLC指纹图谱特征峰总面积、补骨脂素、异补骨脂素、去氢二异丁香酚的含量以及干浸膏得率为综合评判指标。结果:优选出"二神丸"药对最佳醇提工艺:乙醇浓度60%,乙醇8倍用量,每次提取1 h,提取2次。结论:优化的提取工艺稳定、合理、可行,对于规范"二神丸"药对醇提工艺和质量控制具有一定的参考意义。

OBJECTIVE To develop an UPLC fingerprint combined with orthogonal experiment design to optimize the best alcohol extraction process of‘Ershen pills＇ drug pair. METHODS L9（34） orthogonal experiment was used with ethanol concentration,dosage of ethanol,extraction time and extraction times as four factors,UPLC fingerprint characteristic peak area,contents of psoralen,isopsoralen,dehydrodiisoeugenol reference,and dry extract yield as comprehensive evaluation indexes. RESULTS Optimization of the best alcohol extraction process of‘Ershen pills＇ drug pair： 60% ethanol concentration,8 volumes of ethanol,extraction for 2times,1 h for each time. CONCLUSION Optimized extraction process is stable,reasonable and highly efficient,and has certain significance for regulation and quality control of alcohol extraction process of ＂ Ershen pills＂ drug pair.