玉米雄穗结实基因Ts9的初步定位

Initial genetic mapping of a tassel seed gene Ts9 in maize

利用具有Mu9转座子的材料和豫82杂交,从M2分离群体中得到1株雄穗结实突变株,表现型为雄穗上的雄花中雄蕊发育受到抑制,雌蕊不发生退化,雄花转化为雌花。遗传分析表明,该突变性状受1对显性基因控制,初步命名为Ts9。以玉米自交系B73与突变体材料杂交构建BC1分离群体,利用SSR标记将Ts9定位在第1染色体的1.09 bin区域,位于SSR标记umc2047和umc1431之间,距这2个标记之间遗传距离分别为4.2和2.0c M。

From a Mutator inserted M2 segregating population constructed by a cross between Mu9 and Yu 82, a tassel seed mutant was isolated, which shows that the pistil in male flowers on the tassel is not degraded. This makes stamen development restrained and leads to a transformation of tassel florets from stamination to pistillation. The genetic analysis indicated that the mutant trait was controlled by a single dominant gene, designated as Ts9 （tassel seed 9）. In addition,by using SSR markers and BC1 segregating population derived from the cross between the maize inbreds B 73 and the mutant, the Ts9 gene was mapped between umc2047 and umc1431, with the genetic distance of 4.2 cM and 0.8 cM at 1.09 bin of chromosome 1.