柴胡皂苷b_2及柴胡皂苷a在水中的溶解特性研究

Study on the Solubility Characteristics of Saikosaponin b_2 and a in Water

目的考察柴胡皂苷b2、a在水中的溶解特性。方法采用高效液相色谱法测定等体积水中加入不同质量的柴胡皂苷b2、a后的溶解度。色谱条件为：Wonda Sil C18色谱柱（4.6 mm×250 mm,5μm）;流动相：甲醇-水（65∶35）;流速：1.0 ml·min^-1;检测波长：250、210nm;进样量：10.0μl。结果柴胡皂苷b2、a在水中的溶解度随加入浓度的增加均呈现出先升后降的趋势,区别在于柴胡皂苷b2的最大值出现在加入浓度为0.428-0.810 mg·ml^-1范围内。柴胡皂苷a的最大值出现在加入浓度为0.240mg·ml^-1。结论水溶液中的柴胡皂苷处于向水中溶解和自身聚集的动态平衡之中。

Objective To study the solubility characteristics of saikosaponin a and b2 in water solution. Methods The dissolution concentration of saikosaponin at different added concentration of saikosaponin were measured by HPLC method,which was carried out on a Wonda Sil C18column（ 4. 6mm × 250 mm,5μm） with the mobile phase of methanol- water（ 65： 35） with the flow rate at1. 0 ml·min- 1and the wavelength of 210 and 250 nm. Results With the raise of the added concentration of saikosaponin in water the dissolution concentration of saikosaponin firstly increased and then decreased. And the maximum dissolution concentration of saikosaponin b2 appeared at the added concentration of 0. 428 - 0. 810 mg·ml- 1,however that of saikosaponin a was at 0.240mg·ml- 1. Conclusion In the water solution saikosaponin a and b2 are in a dynamic balance of dissolution and aggregation.