摘要
为明确云南万寿菊叶斑病病原菌种属分类地位,对采自云南蒙自的万寿菊叶斑病病叶,依据科赫法则进行分离、纯化、回接和再分离,证明获得的分离物是叶斑病的致病菌。对其形态特征和培养性状进行观察,并对核糖体DNA-ITS序列进行分析。结果表明,致病菌株在PDA培养基上呈灰褐色;分生孢子梗直立或弯曲,有隔膜;分生孢子倒棒形或卵形,有纵、横隔膜,无喙或具短喙。核糖体DNA-ITS序列与GenBank数据库芸苔生链格孢同源性高达100%。将云南万寿菊叶斑病的病原菌鉴定为芸苔生链格孢Alternaria brassicicola。
The pathogens of marigold leaf spot are different at different regions.In order to determine the pathogen of marigold leaf spot in Yunnan Province,Infected leaves were collected from Mengzi,Yunnan Province.The pathogen was isolated,back-inoculated and re-isolated by Koch＇s postulates.According to morphological characteristics,culture shapes and the sequence of ribosmal DNA-ITS.The results showed that the fungus colonies on PDA are grayish-brown,conidiophore erect or ascending,septate,conidia obclavate,obovoid,transverse septa,longitudinal septa,pseudorostra have or not.Ribosomal DNA-ITS region sequence analysis indicated that the fungus pathogen and Alternaria brassicicolaisolate had as high as 100% homology.The pathogen was identified as Alternaria brassicicola.
出处
《西北农业学报》
CAS
CSCD
北大核心
2015年第6期116-119,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
科技部基础工作专项(2006FY120100)
红河学院大学生创新项目(DCXL1306)
云南省高校"农作物优质高效栽培与安全控制重点实验室"建设经费
红河学院硕士点植物保护一级学科建设项目
关键词
万寿菊
叶斑病
芸苔生链格孢
rDNA-ITS序列
鉴定
Marigold
Leaf spot
Alternaria brassicicola
rDNA internal transcribed spacer（ITS）sequence
Identification
