通心络胶囊对糖尿病周围神经病变模型小鼠炎症因子的影响

Effects of Tongxinluo Capsules on Inflammatory Cytokines of Mice with Diabetic Peripheral Neuropathy

目的:研究通心络胶囊对糖尿病周围神经病变模型小鼠炎症因子的影响。方法:将40只KK/Upj-Ay小鼠随机分为模型(纯水)组与通心络低、中、高剂量[1、2、4 g(生药)/kg]组,另选10只C57BL/6小鼠作为对照(纯水)组,ig给药,每天1次,连续12周。末次给药1 h后,测定小鼠痛觉阈值、运动神经传导速度(MNCV)和感觉神经传导速度(SNCV);用流式细胞仪检测血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、环氧合酶2(COX-2)和单核细胞趋化蛋白1(MCP-1)的水平;分别用实时荧光定量聚合酶链反应(RT-PCR)和Western blot法检测小鼠坐骨神经中TNF-α、IL-1β、COX-2、MCP-1 m RNA和蛋白的表达。结果:与对照组比较,模型组小鼠痛觉阈值降低,MNCV、SNCV减慢,血清中TNF-α、IL-1β、COX-2和MCP-1水平升高,坐骨神经中TNF-α、IL-1β、COX-2、MCP-1 m RNA及蛋白的表达增强,差异具有统计学意义(P<0.01或P<0.05)。与模型组比较,通心络中、高剂量组小鼠痛觉阈值升高,MNCV、SNCV加快,血清中TNF-α、IL-1β、COX-2和MCP-1水平降低,坐骨神经中TNF-α、IL-1β、COX-2和MCP-1m RNA及蛋白表达减弱;通心络低剂量组小鼠坐骨神经中COX-2、MCP-1 m RNA及IL-1β、COX-2、MCP-1蛋白表达减弱,差异均有统计学意义(P<0.01或P<0.05)。结论:通心络胶囊对糖尿病周围神经病变模型小鼠有一定的保护作用,其机制可能与抑制炎症因子有关。

OBJECTIVE：To investigate the effects of Tongxinluo capsules on inflammatory cytokines of mice with diabetic peripheral neuropathy.METHODS：40 KK/Upj-Ay mice were randomly divided into model group（pure water）,and Tongxinluo low,medium and high dose groups [1,2 and 4 g（medicinial materials）/kg].10 C57BL/6 mice were selected into the control group（pure water）.The drugs were given once a day for consecutive 12 weeks,ig.The pain perception threshold,motor nerve conduction velocity（MNCV）and sensory nerve conduction velocity（SNCV）were detected for the mice 1 h after the last administration of drugs.The flow cytometry was used to detect the levels of tumor necrosis factor α（TNF-α）,interleukin-1β（IL-1β）,cyclooxygenase 2（COX-2）and monocyte chemoattractant protein 1（MCP-1）in serum.Real-time fluorescent quantitative polymerase chain reaction（RT-PCR）and Western blot were respectively employed to detect the m RNA and protein expressions of TNF-α,IL-1β,COX-2 and MCP-1 in the mice＇s sciatic nerves.RESULTS：Compared with control group,pain perception threshold in model group was decreased;MNCV and SNCV were slowed;the levels of TNF-α,IL-1β,COX-2 and MCP-1 in serum were increased;the expressions of m RNA and protein of TNF-α,IL-1β,COX-2,MCP-1 were increased,with significant differences（P0.01 or P0.05）.Compared with model group,pain perception threshold in Tongxinluo medium and high dose groups were increased;MNCV and SNCV were increased;the levels of TNF-α,IL-1β,COX-2 and MCP-1 in serum were decreased;the expressions of m RNA and protein of TNF-α,IL-1 β,COX-2,MCP-1 in sciatic nerves were decreased;the expressions of m RNA of COX-2,MCP-1 and protein of IL-1β,COX-2,MCP-1 in sciatic nerves in Tongxinluo low dose group were decreased,there were statistical significant difference（P0.01 or P0.05）.CONCLUSIONS：Tongxinluo capsules have certain protective effects on the mice with peripheral nerve in case of diabetes by a mechanism that may be associated with the inhibition of inflammatory cytokines.