黄芩提取物对阿尔茨海默病模型小鼠的抗氧化作用

Antioxidant Effects of Extracts from Scutellaria baicalensis on Model Mice with Alzheimer's Disease

目的:研究黄芩提取物(SBE)对阿尔茨海默病(AD)模型小鼠的抗氧化作用。方法:ip给予D-半乳糖(150 mg/kg)和亚硝酸钠(100 mg/kg),每天1次,连续60 d以复制小鼠AD模型。60只小鼠随机均分为正常对照(等容氯化钠注射液)组、模型(等容氯化钠注射液)组、雌二醇(0.4 mg/kg)组与SBE高、中、低剂量(500、250、125 mg/kg)组,除正常对照组外,其余各组复制模型同时ig给药,每天1次,连续60 d。测定小鼠脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量,以及血清中谷胱甘肽过氧化物酶(GSH-Px)活性。结果:与正常对照组比较,模型组小鼠脑组织SOD、CAT活性减弱,MDA含量增加,血清中GSH-Px活性减弱,差异有统计学意义(P<0.01或P<0.05);与模型组比较,SBE高、中剂量组小鼠脑组织SOD、CAT活性增强,MDA含量减少,血清中GSH-Px活性增强,差异有统计学意义(P<0.01或P<0.05)。结论:SBE可增强AD模型小鼠抗氧化能力,其机制可能与改善脑组织与血清中相关抗氧化指标水平有关。

OBJECTIVE：To study the antioxidant effects of Scutellaria baicalensis（SBE）on the model mice with Alzheimer＇s disease.METHODS：All mice were treated by D-gal（150 mg/kg）and Na NO2（100 mg/kg）for 60 d to reproduce the AD model mice,ip,once a day.Totally 60 mice were randomly divided into normal control group（isovolumetric sodium chloride injection）,model group（isovolumetric sodium chloride injection）,estradiol（0.4 mg/kg） group,and SBE high,medium and low doses（500,250 and 125 mg/kg）.The mice were intragastrically administrated while modeling except normal control group,once a day,for continuous 60 d.The superoxide dismutase（SOD）,catalase（CAT）activities and malondialdehyde（MDA）content in brain tissue,and glutathione peroxidase（GSH-Px）activity in serum of mice were determined.RESULTS：Compared with normal control group,the SOD,CAT activities in brain tissue,and GSH-Px activity in serum of mice in model group were decreased,MDA content in brain tissue were increased,with significant difference（P〈0.01 or P〈0.05）;compared with model group,the SOD,CAT activities in brain tissue and GSH-Px activity in serum of mice in SBE high and medium dose groups were increased,MDA content was decrease,with significant difference（P〈0.01 or P〈0.05）.CONCLUSIONS：SBE can enhance the antioxidant capacity in AD model mice by a mechanism that may be related to the improvement of related oxidation indicators＇ levels.