臭氧自体血治疗痛风缓解疼痛的细胞机制研究

Cellular Mechanism of Pain Relief by Ozonated Autohemotherapy in Treatment of Gouty Patients

目的观察痛风患者臭氧自体血治疗前后血清免疫球蛋白水平及炎性细胞因子水平的改变,探讨其可能的细胞作用机制。方法选择2012年6月—2013年9月首都医科大学宣武医院就诊的10例男性痛风患者,均采用臭氧自体血治疗,20 mg/L,3次/周,共10次。采集治疗前、第5次治疗后和第10次治疗后的静脉血,采用免疫比浊法检测血清Ig A、Ig M、Ig G和补体C3、C4水平。采用流式细胞微球阵列分析方法 (CBA)检测患者治疗前后血清IL-8、IL-12、单核细胞趋化蛋白1(MCP-1)水平。结果患者治疗前后Ig A、Ig M、Ig G、补体C3、补体C4、IL-12、MCP-1水平比较,差异无统计学意义(P>0.05)。患者治疗前后IL-8水平比较,差异有统计学意义(P<0.05);其中,患者第5次治疗后和第10次治疗后IL-8水平均低于治疗前(P<0.05)。结论 IL-8可能是诊断和治疗痛风的新标志物和未来治疗痛风的研究对象,但臭氧自体血治疗痛风缓解疼痛的确切机制仍有待进一步研究。

Objective To investigate the changes of serum immunoglobulin and cytokine levels before and after ozonated autohemotherapy in the treatment of gouty patients and to study its possible cellular mechanism. Methods Ten male gouty patients who were admitted to Xuanwu Hospital,Capital Medical University were treated with ozonated autohemotherapy using an ozone concentration of 20 mg/ L. The patients were treated three times a week for a total of ten times. Samples of venous blood serum were collected before treatment,after five times of treatment and ten times of treatment respectively. Serum IgA,IgM,IgG and serum cytokines（C3 and C4 ）were detected by immunoturbidimetry and serum IL - 8,IL - 12 and MCP - 1 were detected by cytometric bead arrays. Results The IgA,IgM,IgG,C3 ,C4 ,IL - 12 and MCP - 1 levels showed no statistically significant differences before and after treatment（ P 〉 0. 05）;the IL - 8 level before and after treatment showed statistically significant difference（P 〈 0. 05）. After ten times of treatment and five times of treatment,the IL - 8 level was significantly lower than that before treatment（ P 〈 0. 05）. Conclusion IL - 8 may be a new marker of diagnosis and treatment and a future research target in the treatment of gout. The exact mechanism of pain relief by ozonated autohemotherapy in the treatment of gouty patients still remains to be studied.