粗毛火炭母化学成分的研究

Chemical Constituents of Whole Plant of Polygonum chinese L.var hispidum Hook.f.

目的研究粗毛火炭母的抗氧化活性及化学成分,为确定其质量控制指标性成分提供依据。方法利用氧自由基吸收能力(oxygen radical absorption capacity,ORAC)测定方法评价粗毛火炭母乙醇提取物不同部位的抗氧化活性。采用硅胶、Sephadex LH-20凝胶、MCI、半制备HPLC等手段进行分离纯化,并通过1H-NMR,13C-NMR,MS等波谱鉴定结构。结果乙酸乙酯部位具有较好的抗氧化活性,并从石油醚和乙酸乙酯部位分离鉴定了15个化合物,分别为异补骨脂素(1),2,3,4,6,7-五甲氧基-9,10-二氢菲(2),1,5,7-三羟基-3-甲基蒽醌(3),槲皮素(4),没食子酸甲酯(5),没食子酸(6),正二十一烷酸(7),正十九烷酸(8),花生酸(9),三十烷酸(10),正二十四烷酸(11),棕榈酸(12),正三十二烷酸(13),β-谷甾醇(14)和胡萝卜苷(15)。结论粗毛火炭母乙醇提取物不同部位均具有一定的抗氧化活性,但乙酸乙酯部位抗氧化活性最好。所有化合物均为首次从该植物中分离得到,其中化合物2为首次从该属植物中分离得到。

OBJECTIVE To investigate the antioxidant activities and chemical constituents of Polygonum chinese L. var hispidum Hook. f. METHODS The compounds were isolated by chromatographic techniques with silica gel,Sephadex LH- 20 and semi-preparative HPLC. Their structures were identified by analysis of physical and spectral data,and confirmed by comparison of their spectral data with the reported values in the literature or those of authentic samples. The antioxidant activities of the extractions of Polygonum chinese L. var hispidum Hook. f. were evaluated by oxygen radical absorption capacity（ ORAC） assay. RESULTS Fifteen compounds were isolated and identified as isopsoralen（ 1）,2,3,4,6,7-pentamethoxy-9,10-dihydrophenanthrene（ 2）,1,5,7-tri hydroxy-3-methylanthraquinone（ 3）,quercetin（ 4）,gallincin（ 5）,gallic acid（ 6）,heneicosanoic acid（ 7）,nonadecanoic acid（ 8）,arachidic acid（ 9）,triacontanoic acid（ 10）,tetracosanoic acid（ 11）,palmitic acid（ 12）,lacceroic acid（ 13）,β-sitosterol（ 14）,and daucosterol（ 15）,respectively. Ethyl acetate extracts had stronger antioxidant activities. CONCLUSION All compounds are isolated from the species for the first time,and compound 2 is obtained from this genus for the first time