哺乳动物雷帕霉素靶蛋白及其底物激酶在肾癌中的表达及临床意义

Expression of mammalian mTOR and P70S6K in renal cell carcinoma and its clinical significance

目的探讨雷帕霉素靶蛋白（mTOR）和底物激酶（P70S6K）蛋白在肾癌组织中的表达，分析其在肾癌发展中的意义。方法以40例肾癌组织标本、40例正常肾组织标本为对象，行免疫组化SP及Westernblotting法测定组织中mTOR、P70S6K含量，分析其表达与肾癌临床病理参数关系。结果mTOR、P70S6K蛋白在肾癌组织中均呈高表达，正常肾组织呈低表达，对比差异有统计学意义（P〈0．05）。两者在肾癌组织表达与细胞分化程度、淋巴结转移等显著相关（P〈0．05），与年龄、性别无显著相关（P〉0．05）。两者在肾组织中的表达呈正相关（P〈0．05）。结论mTOR、P70S6K在肾癌组织中呈阳性高表达，与细胞分化、淋巴转移等相关。

Objective To explore the expression of mTOR and P70S6K in kidney cancer tissue and analyze its significance in the development of renal cancer. Methods 40 renal carcinoma tissue specimens and 40 normal renal tissue specimens were set as study objects. Immunohistochemical SP and Western blotting method were used to detect the content of mTOR and P70S6K in the tissue.The relationship between expression and kidney cancer clinical pathological parameters was analyzed. Results mTOR and P70S6K more highly expressed in kidney cancer tissue than in normal renal tissue, with statistical differences （P 〈 0.05）. The expressions of mTOR and P70S6K in kidney cancer tissue closely correlated with the degree of cell differentiation and lymph node metastasis （P 〈 0.05）, but not with age and sex （P 〉 0.05）. The expression of mTOR positively correlated with the expression of P70S6K in renal tissue （P 〈0.05）. Conclusions The expressions ofmTOR and P70S6K are positively high and correlate with cell differentiation, lymph node metastasis, etc.