自然发酵蔬菜制品中降解亚硝酸盐乳酸菌的分离与鉴定

Isolation and identification of nitrite-degrading lactic acid bacteria from natural fermented vegetable

以自然发酵蔬菜为原料,采用平板计数和钙溶圈法分离纯化菌株,并分析菌株的亚硝酸盐降解能力。结果表明,钙溶圈法分离的产酸能力较强的9株细菌,经生理生化鉴定均为乳酸菌。乳酸菌在MRS培养基中都具有亚硝酸盐分解能力,其中H12、L7和X1三株乳酸菌亚硝酸盐降解率高于89%,且降解速度较快,其中X1降解亚硝酸活性最强。乳酸菌X1还表现良好的产酸能力和耐盐性,经16S r DNA鉴定为植物乳杆菌(Lactobcillus plantarum)。

Bacteria with high nitrite degradation ability were isolated from natural fermented vegetable by plate count method and calcium dissolved ring method. Nine strains with strong acid production ability were screened and identified as lactic acid bacteria by physiological and biochemical identification. The nitrite degradation ability in MRS liquid medium differed among nine strains, strain H12, L7 and X1 exhibited rapid nitrite degradation rate of higher than 89%. Strain X1 showed the strongest ability for nitrite degradation, and it also showed strong ability of acid production and salt resistance, which was identified as Lactobcillus plantarum by 16S rDNA sequencing.