母婴分离联合束缚应激幼鼠IBS-D模型的建立及评价

Establishment and evaluation of rat model of diarrhea predominant irritable bowel syndrome with the method of neonatal maternal separation plus restraint stress

[目的]采用母婴分离联合束缚应激法建立腹泻型肠易激综合征大鼠模型,并对其进行初步评价。[方法]刚出生1d的SD幼鼠随机分为4组:对照组(NH),母婴分离组(NMS),束缚应激组(RS),母婴分离联合束缚应激组(NMS+RS)。造模结束后,分别观察其体重变化、排便情况及腹壁回撤反射(AWR)。[结果]模型组大鼠体重均较NH组低(P<0.01),且二因素组(NMS+RS组)较单因素组(NMS组,RS组)更低,差异均有统计学意义(P<0.05)。大鼠粪便Bristol分级评分及粪便含水量,模型组均较NH组高,结果有统计学意义(P<0.05);二因素组较单因素组高,差异有统计学意义(P<0.01)。在10mmHg及20mmHg时,4组之间腹壁回撤反射(AWR)评分结果近似,差异无统计学意义(P>0.05);40~80mmHg时,NMS组,NMS+RS组AWR评分均较NH组高,差异有统计学意义(P<0.05);60mmHg及80mmHg时,NMS+RS组AWR评分较RS组高,二者结果差异有统计学意义(P<0.05)。[结论]母婴分离法、束缚应激法均能在一定程度上模拟腹泻型肠易激综合征的临床症状及(或)内脏高敏感性,采用二者相结合(母婴分离联合束缚应激)的方法建立的大鼠模型与任何单一方法比较,更加符合腹泻型肠易激综合征的症状及内脏高敏感性的特征。

[Objective]To establish rats model of diarrhea predominant irritable bowel syndrome with the method of neonatal maternal separation plus restraint stress, and to conduct the preliminary evaluation on it. [Methods]One day newborn SD rats were randomly divided into 4 groups： control group （NH）, neonatal maternal separation group （NMS） and restraint stress group （RS）, neonatal maternal separation plus restraint stress group （NMS＋RS）. After the modeling, the weight change, defecation and abdominal withdrawal reflex （AWR） were observed respectively. [Results]On weight, NMS group, RS group, NMS ＋RS group were lower than that of NH group （P〈0.05）;And, the double factor group （NMS＋RS group）, compared with single factor group （NMS, RS）, was lower and the results were statistically different （P〈0, 05）. Bristol Stool Scale and waste water content （%） in the NMS group, RS group, NMS＋ RS group were all higher than that in NH group, the results were statistically different （P^0.05）. Doub- le factor group in Bristol Stool Scale and waste water content were higher than that in single factor groups, the difference was statistically significant （P〈0.01）. The scores of AWR between 10 mmHg and 20 mm-Hg in the four groups were insignificantly different （P〉0.05） ; When between 40mmHg and 80 mmHg, the scores of AWR in NMS group and NMS＋RS group were all higher than that in the NH group, the results had statistical significance （P〈0.05） ; While the RS group compared with NH group, the results had no significant difference （P〉0.05）. When between 60 mmHg and 80 mmHg, the scores of AWR in NMS ＋RS group was higher than that in RS group, the results were statistically different （P〈0.05）. l-Conclusion]The method of neonatal maternal separation and restraint stress could simulate clinieal symptoms of diarrhea-predominant irritable bowel syndrome and （or） high internal sensitivity to a certain extent. To establish the rat model, the combination of the two methods （neonatal maternal separation plus restraint stress） was more efficient than the single use of each on inducing the symptoms of diarrhea-predominant irritable bowel syndrome and the characteristic of high internal sensitivity. Key words：