免疫亲和柱净化-柱后衍生高效液相色谱荧光法快速检测玉米粉中黄曲霉毒素B_1

Determination of Aflatoxin B_1 in Corn Flour by Immune Affinity Column Purification and HPLC-FLD with Post-Column Photochemical Derviatization

本文建立了免疫亲和柱净化-高效液相色谱荧光法-陕速检测玉米粉中黄曲霉毒素B_1(aflatoxin B_1,AFB1)的方法。样品经甲醇/水(4∶1.v/v)浸提,免疫亲和柱净化,高效液相色谱分离,光化学柱后衍生,荧光检测器测定。结果表明:AFB_1在2.5~150μg/L具有良好的线性关系,相关系数为R^2=0.9998,检测限为1.0μg/kg,平均回收率为84.0%~96.3%,RSD范围为0.7%~2.5%。该方法具有快速、准确和重复性好的优点,适用于大批次玉米及玉米粉制品中AFB1的检测。

A method for determination of aflatoxin B1 （AFBI） in corn flour using immune-affinity column purification and HPLC with post column photo-chemical derivatization was developed. Samples were extracted with methanol-water solution （4：1, v/v）, and purified by immune-affinity columns. Seperation of AFBI were conducted by HPLC and determination was carried out by fluorescence detector after photochemical derivatization. Results showed that this method had good linearity range from 2.5-150 μg/L, R^2=0.9998, detection limit was 1.0μg/kg, recoveries of analytes were from 84.0% -96.3% , RSD was 0.7% -2.5%. Method is quick, precise, high sensitive and good repeatability, which is suitable for the determination of AFBI in corn flour.