HPLC-ELSD法测定低聚木糖中的木糖和木二糖

Determination of Xylose and Xylobiose in Xylo-oligosaccharide by HPLC-ELSD

建立了高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定低聚木糖中的木糖和木二糖含量的方法。以Prevail TM Carbohydrate ES糖柱（250×4.6,5μm）为色谱柱,乙腈—水（72∶28,V/V）为流动相,流速为1.0 m L/min,柱温为25℃,进样量为10μL,ELSD漂移管温度为80℃,载气流速为2.0 L/min。结果表明,木糖和木二糖分别在0.08～1.00 mg/m L和0.15～2.00 mg/m L质量浓度范围内线性关系良好,检出限分别为0.01mg/m L和0.02 mg/m L,定量限分别为0.03 mg/m L和0.05 mg/m L,样品加标回收率为97%～102%。

An analytical method was established for the determination of xylose and xylobiose by HPLC-ELSD. The optimal conditions were as follows： column was PrevailTM Carbohydrate ES （250×4.6, 5 μm）, mobile phase： mixture of acetonitrile and water （72 ： 28）, flow rate： 1.0mL/min, column temperature： 25 ℃, injection volume： 10 μL, drift tube temperature： 80 ℃, flow rate of carrier gas： 2.0 L/min. Under the optimal conditions, the calibration curve was linear over the content of xylose and xylobiose in the range of 0.08-1.00 mg/mL and 0.15-2.00 mg/mL, the detection limits （LOD） were 0.01 mg/mL and 0.02 mg/mL, the quantification limits （LOQ） were 0.03 mg/mL and 0.05 mg/mL, respectively. The spiked recoveries of xytose and xylobiose were 97 %-102 %.