血清高尔基体膜蛋白73在原发性肝癌早期诊断中的应用价值

The Clinical Value of Serum Golgi protei73 in Making Early Diagnosis of Primary Hepatocellular Carcinoma

目的探讨原发性肝癌血清中高尔基体膜蛋白-73的表达水平及其早期诊断价值。方法选择住院患者和健康体检人群,分别用酶联免疫吸附法和化学发光免疫分析法检测63例原发性肝癌、56例肝硬化、87例慢性乙型肝炎、48例其他恶性肿瘤、58例健康对照组血清高尔基体膜蛋白-73与AFP水平,分析两指标各组间差别,绘制其诊断原发性肝癌的ROC曲线。结果高尔基体膜蛋白-73在不同年龄、性别、肿瘤大小间及不同AFP浓度的表达差异无统计学意义,高尔基体膜蛋白-73、AFP在PHC鉴别诊断的ROC曲线下面积分别为0.808、0.758。高尔基体膜蛋白-73诊断PHC组敏感性及特异性分别为88.6%,92.1%,均显著高于AFP。结论血清高尔基体膜蛋白-73对原发性肝癌的诊断价值优于AFP,有望成为原发性肝癌早期诊断的血清标记物。

Objective To investigate the expression level of serum Golgi protein 73（GP73）in patients with primary hepatocellular carcinoma（HCC）and to discuss its clinical value in making early diagnosis. Methods Among wards,out-patients and healthy check people in our hospital from October 2012 to October 2014, 63 HCC, 56 hepatic cirrhosis,87 HBV carriers,48 other Malignant Tumor,and 58 healthy control people were involved in the study. GP73 in serum was measured by ELISA and AFP was measured by ADVIA Centaur XP Immunoassay System.The sensitivity,specification were calculated. Results The serum levels of GP73 in different ages, gender, tumor sizes and concentriations of AFP was not significantly different. The area of AFP, GP73 under the ROC curve in the differential diagnosis of PHC were0.758, 0.808. The sensitivity and specificity value of GP73 were 88.6% and 92.1%, higher than those of AFP. Conclusion In diagnosing HCC,the expression level of serum GP73 is superior to AFP. It is hopeful that serum GP73 may be proved to be an early serum tumor marker for HCC.