参附注射液对胆汁环境中成纤维细胞的影响

Study of Shenfu injection on fibroblasts in bile environment

目的探讨在胆汁环境中参附注射液对成纤维细胞的影响,以判断在此环境中成纤维细胞的增殖是否能被参附注射液抑制,从而减轻胆道瘢痕性狭窄。方法通过建立体外胆汁环境中成纤维细胞生长模型,运用参附注射液干预。设计分为空白组、胆汁组、实验组,实验组又分为5个浓度梯度亚组。运用MTT法测定各组中成纤维细胞OD值,用SPSS20.0统计软件处理,分析参附注射液是否能抑制成纤维细胞的形成。结果成纤维细胞OD值随参附注射液含量及干预时间的增加逐渐减小(P<0.05),差异具有统计学意义。说明在胆汁环境中成纤维细胞的增殖能被参附注射液抑制。结论参附注射液对胆汁环境中成纤维细胞的增殖有抑制作用。

Objective To analyze the effect of Shenfu injection on fibroblasts in bile environment in order to judge whether fibroblast proliferation could be inhibited by Shenfu injection to reduce biliary stricture.Methods Fibroblasts growth model was established in bile environment,which was intervened by Shenfu injection.We divided the groups into the blank control group,bile group and experimental groups that was divided into 5 subgroups according to the concentrations.We calculate OD value of the fibroblasts by the MTT method to analyze whether Shenfu injection could inhibit the formation of fibroblasts.We used SPSS20.0 software for statistical analysis.Results The OD values decreased gradually with Shenfu injection concentration and intervention time（P 〈0.05）.Conclusion In bile environment,the fibroblasts could be inhibited by Shenfu injection.