PGE_1预处理对缺血再灌注心肌NF-κB及ICAM-1表达的影响

Effects of Prostaglandin E_1 on NF-κB and ICAM-1 expression in preconditioning myocardium with ischemia/reperfusion

目的探讨PGE1预处理对缺血再灌注损伤心肌NF-κB和ICAM-1表达的影响,进一步从炎症信号通路揭示PGE1对MIRI保护作用的可能机制。方法 40只大鼠随机分为5组:正常对照组、缺血再灌注组(I/R组)、PGE114μg/L预处理组、PGE142μg/L预处理组和PGE1126μg/L预处理组。采用Langendorff大鼠离体心脏灌流装置制备MIRI模型,制备病理切片观察大鼠心肌组织形态学的变化,免疫组化法测定NF-κB及ICAM-1的表达。结果 PGE1各剂量预处理组大鼠缺血再灌注心肌组织形态学变化得到不同程度的改善;NF-κB及ICAM-1的表达在各PGE1预处理组较I/R组明显减少,且各组之间比较有统计学差异(P<0.05)。结论 PGE1预处理可以改善大鼠缺血再灌注心肌组织形态学变化,并通过抑制NF-κB和ICAM-1的表达减轻心肌中PMN介导的炎症反应,有效减轻离体大鼠MIRI。

Objective To investigate the effects of NF-κB and ICAM-1expression in preconditioning myocardium with ischemia reperfusion injury and reveal the possible mechanism of PGE1 protective effect from inflammatory signaling pathways.Methods 40 rats were divided into five groups at random：normal control group,ischemia/reperfusion group and PGE1 14,42,126μg/L preconditioning groups.The model of myocardial ischemia reperfusion injury was established in isolated rat heart perfused with Langendorff technique,the pathological slices were maked to observe the pathomorphological changes of the rat myocardial fibers,the immunohistochemical method was used to measure the changes of NF-κB and ICAM-1expression.Results The morphological changes of ischemia/reperfusion myocardial fiber were improved in PGE1 preconditioning groups.Compared with the I/R group,the myocardial expression of NF-κB and ICAM-1in PGE1 preconditioning group was significantly reduced（P〈 0.05）.Conclusion PGE1 preconditioning can improve the morphological changes of ischemia/reperfusion myocardial fibre,inhibit the expression of NF-κB and ICAM-1in order to effectively reduce the myocardial inflammation mediated PMN and reduce ischemia/reperfusion injury in isolated rat heart.