脑心通对高脂饲养ApoE^(-/-)小鼠动脉粥样硬化的影响及机制研究

Effect of Naoxintong on Atherosclerosis in Apo E^-/- Mice Fed a High-fat Diet

目的探究脑心通对高脂喂养Apo E-/-小鼠动脉粥样硬化的影响并初步阐明其作用机制。方法 29只8周龄Apo E-/-雄性小鼠随机分为高脂组(n=11)、高脂加匹伐他汀组(3mg/kg/d,n=7)和高脂加脑心通组(0.7g/kg/d,n=11),给予高脂饲料(20%猪油,0.5%胆固醇)喂养9周后处死,检测血浆中甘油三酯(TG)、总胆固醇(TC)和高密度脂蛋白胆固醇(HDL-C)水平;取主动脉进行油红O染色观察主动脉弓斑块生长情况;制作主动脉根部冰冻切片,分别进行油红O、Masson染色,检测主动脉根部斑块面积和胶原含量;ELISA方法检测血浆中炎症因子IL-1β水平。结果脑心通和匹伐他汀组小鼠血浆TC略低于高脂组,但未达统计学意义(P均>0.05),各组之间TG无显著差异(P>0.05);油红O染色结果显示,脑心通组及匹伐他汀组主动脉弓处斑块占主动脉弓比例显著低于高脂组(P<0.05),脑心通和匹伐他汀组主动脉根部斑块面积较高脂组下降,但未达统计学差异(P>0.05);主动脉根部斑块Masson染色结果显示,脑心通组小鼠主动脉根部斑块中胶原比例显著高于高脂组(P<0.05);ELISA结果显示,脑心通组小鼠血浆IL-1β水平较高脂组显著下降(P<0.05)。结论脑心通能够降低高脂饲养Apo E-/-小鼠血浆IL-1β水平,减少小鼠主动脉弓处斑块形成,增加主动脉根部斑块中胶原含量。

Objective To assess the effect of Naoxintong on atherosclerosis in ApoE^-/- mice. Methods Twenty-nine eight-week-old male ApoE^-/- mice were randomly divided into 3 groups： high-fat group （n=11）, pitavastatin group （n=7） or Naoxintong group （n=11）.Then the mice were ted with high-fat diet for 9 weeks. After 9 weeks of diet, mice were sacrificed, blood was collected and plasma TG, TC and HDL-C concentrations were measured. Aortic arch plaques were stained with oil red O and plaque area was analyzed. Atherosclerosis plaque in aorta root was made into frozen sections, plaque area and collagen content was analyzed by oil red O and Masson staining, respectively. Plasma IL-1β concentration was detected by ELISA. Results Plasma levels of TC and HDL-C in the Naoxintong group slightly decreased compared with those in the high-fat group （P〉0.05）, and TG showed no significant change among the three groups （P〉0.05）.Oil red staining showed the ratio of the lesion area in aortic arch was significantly smaller in Naoxintong group and pitavastatin group than in high-fat group（P〈0.05）,and the lesion area of aortic sinus was slightly smaller in Naoxintong group and in pitavastatin group compared to high-fat group, but did not reach statistical difference （P〉 0.05）;Masson staining showed collagen content in Naoxintong group significantly increased compared with high-fat group（P〈0.05）.ELISA results showed decreased plasma IL-1β concentration in Naoxintong group compared with high-fat group（P〈0.05）.Conclusion Naoxintong may reduce the levels of plasma 1L-1β, and protects against atherosclerosis in ApoE^-/- mice fed a high-tat diet. In addition, Naoxintong may accelerate collagen deposition, which could contribute higher aorta root plaque stability.