‘陇薯3号’和‘陇薯7号’试管结薯关键条件优化

Optimization of Key Conditions to Induce Potato Microtuber of'Longshu 3' and 'Longshu 7'

应用不同光照、温度、培养基等条件诱导‘陇薯3号’和‘陇薯7号’试管结薯,结果表明,自然光照室温培养、自然光照室温培养7 d转黑暗恒温培养以及黑暗恒温培养条件均能诱导2个品种结薯。自然光照室温条件下,‘陇薯7号’试管薯单瓶薯数、单瓶薯重及大薯数均极显著高于其他培养条件(P<0.01)。与"固体+液体"、"固体"培养基相比,"液体+液体"培养基结薯时间早,数量多,大薯率高。

The inducing trials of potato microtubers ＇Longshu 3＇, and ＇Longshu 7＇ were carried out under different culture conditions of light, temperature and medium. The results showed that the two varieties could form microtubers under the culture condition of natural lighting and room temperatures, or darkness and constant temperature, or switching to darkness and constant temperature after seven days of natural lighting and room temperatures culture. Tuber number per bottle, tuber weight per bottle and big tuber number of ＇Longshu 7＇ under the condition of natural lighting and room temperatures were significantly higher than other conditions（P〈0.01）. ＂Liquid ＋ liquid＂ medium produced more and heavier microtubers, and also higher rate of big potato and earlier microtuber formation than ＂solid ＋ liquid＂ medium and ＂solid＂ medium.