内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响

Effects of Endogenous n-3 Polyunsaturated Fatty Acids(PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model

利用fat-1转基因小鼠模型,研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用.采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周,然后2组小鼠给予高糖饮液自由饮用4周,每周测体重.第8周末,取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平.结果表明:fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05).认为n-3 PUFAs能抑制体重增长,降低小鼠血糖、血脂和胰岛素抵抗,起到调节血糖、血脂的作用.

In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10% corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride （TG）, serum total cholesterol （TC）, serum high-density lipoprotein cholesterol （HDL-C）, serum low-density lipoprotein cholesterol （LDL-C） levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TC~ HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice （P〈0.05）. The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance.