摘要
研究了二氧化钛(Ti O2)对蛋黄蛋白中卵黄高磷蛋白磷酸肽(PPPs)的纯化性能,确定了最优纯化工艺,并对PPPs产品的持钙性能进行了研究。结果表明,在p H 4.0,吸附时间20 min,粗蛋黄多肽初始质量浓度为40mg/m L,反应温度为25℃时,Ti O2可以从粗蛋黄水解多肽中纯化得到纯度较高(N/P为5.34)且具有显著延缓钙离子沉淀效果的PPPs。同时Ti O2还具有较强的稳定性,可实现重复利用。
In order to determine the purification performance of titanium dioxide（ TiO2） to phosvitinphosphopeptides（ PPPs）,we investigated the optimum conditions of PPPs purification and inhibiting ability on calcium precipitation. The results showed that the optimum condition for PPPs purification was as follows： p H 4. 0,time 20 min,initial peptides concentration 40 mg / m L,reaction temperature 25 ℃. The purity of the optimized PPPs was high（ N / P5. 34） and the effect of inhibiting calcium precipitation was obvious. The study not only provided a new way for the comprehensive utilization of defatted egg yolk protein but also provided a new research direction for the purification of PPPs.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第12期26-31,共6页
Food and Fermentation Industries
基金
国家高技术研究发展计划(863计划)(No.2013AA102207)
