HLA-B特异性抗原Bw4与新疆汉族鼻咽癌的相关性研究

The association between HLA-B antigen carrying Bw4 epitope and nasopharyngeal carcinoma among Han people in Xinjiang

目的探讨HLA-B特异性抗原Bw4与新疆汉族鼻咽癌发病的关系。方法应用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对131例汉族鼻咽癌患者(鼻咽癌组)和168例汉族健康体检者(对照组)进行HLA-B位点等位基因分型,确定Bw4、Bw6抗原的特异性分布,分析HLA-B携带Bw4对鼻咽癌患者CD4+、CD8+T淋巴细胞亚群的影响。结果 (1)鼻咽癌组Bw4抗原携带者66例(50.4%),健康对照组Bw4抗原携带者120例(71.4%),鼻咽癌组Bw4抗原表达频率明显低于健康对照组,两组比较差异有统计学意义(χ2=13.869,P=0.000)。(2)鼻咽癌组CD4+T淋巴细胞水平低于健康对照组,CD8+T淋巴细胞水平高于健康对照组,两组比较差异均有统计学意义(t=2.765,P=0.009;t=-1.768,P=0.043)。(3)鼻咽癌患者中Bw4阳性组CD4+T淋巴细胞水平高于Bw4阴性组,两组比较差异有统计学意义(t=3.002,P=0.005),两组CD8+T淋巴细胞水平比较差异无统计学意义(P>0.05)。结论 Bw4抗原的缺失可能与新疆汉族鼻咽癌的发生有关,HLA-B特异性抗原Bw4对汉族鼻咽癌患者有一定的保护作用。

Objcetive To investigate the relationship between human leukocyte antigen-B（HLA-B）antigen carrying Bw4 and Han people with nasopharyngeal carcinoma （NPC）in Xinjiang.Methods Polymerase chain reaction-sequence specific primers （PCR-SSP）technique was employed to detect the allele frequency of HLA-B in 131 Han NPC patients and 168 normal controls.HLA-Bw genotypes were determined and their association with CD4 ＋ T cell,CD8 ＋ T cell were analyzed.Results （1）There were 66 （50.4%） cases with Bw4 in NPC,and 120（71.4%）cases with Bw4 in normal controls.The population frequency of Bw4 in NPC was lower than normal controls （χ2 =13.869,P =0.000）.（2）The percentage of CD4＋ T cell in Han NPC was lower than normal controls （t =2.765,P =0.009）and the percentage of CD8＋ T cell in Han NPC was higher than normal controls （t =-1.768,P =0.043）.（3）In NPC,the group with Bw4 had higher percentage of CD4＋ T cell compared with the group without Bw4 （t =3.002,P =0.005）and there was no significant differences in the percentage of CD8＋ T cell between the two groups （P 〉0.05）.Con-clusion Bw4 deficiency was associated with Han NPC in Xinjiang.HLA-B antigen carrying Bw4 has pro-tective effect in Han NPC.