子午流注针刺法对哮喘模型豚鼠气道重塑的干预作用

Effects of Midnight-noon and Ebb-flow Acupuncture Therapy on Airway Remodeling in Asthmatic Guinea Pigs

目的探讨纳子法对哮喘豚鼠模型气道重塑的针灸治疗效果,揭示子午流注纳子法针对哮喘气道重塑的机理,为临床运用子午流注治疗哮喘提供一定的实验依据。方法将32只健康豚鼠分为A组于申时(15:00-17:00)进行电针,B组于巳时(9:00-11:00)进行电针,C组造模不治疗和D组(正常对照组),每组8只,并采用鸡卵白蛋白致敏法复制实验性过敏性哮喘模型,以此观察4组豚鼠的气道壁和气道平滑肌厚度及肺组织转化生长因子(TGF)-β1含量的变化情况。结果 A、B组与C组比较,肺内气管壁厚度、平滑肌厚度以及TGF-β1含量均明显降低(P<0.01);且A组比B组更能有效降低厚度及TGF-β1含量。结论电针治疗能阻止哮喘气道重塑的发生发展,并且按时辰取穴的纳子针法申时治疗A组的哮喘气道重塑改善情况优于不按时辰治疗的巳时B组,提示按时辰针灸对哮喘疗效更佳。

Objective： To observe the Midnight-noon and Ebb-flow Acupuncture therapeutic effect on airway remodeling of asthma guinea pig model and to reveal the mechanism of it which provided experimental basis for clinical application of the Midnight-noon Ebb-flow Acupuncture in treatment of asthma. Methods： 32 healthy guinea pigs were divided into A group with electroacupuncture in Shen shi group（from 15：00 to 17：00 PM）,the B group with electroacupuncture in Si shi Group（from 9：00-11：00）,the C group with no treatment and he D group with normal control therapies. And chicken ovalbumin sensitization method was used to copy the model of experimental asthma to observe the 4 groups of guinea pig airway thickness and airway smooth muscle and the change of TGF-β1content in lung tissue. Results： Compared with the C group,intrapulmonary airway wall thickness,smooth muscle thickness and the content of TGF-β1were decreased significantly in the A group and B group（P 0.01）. And the reductions of thickness and TGF-β1in the A group were more effectively than those in the B group. Conclusion： Electroacupuncture treatment can prevent the occurrence and development of airway remodeling in asthma. It is better efficacy on treating asthma on time that the time points of the Shen shi group of airway remodeling in asthma were better than those of no in accordance with the hour treatment in Si shi group.