苦酸通调方对2型糖尿病大鼠胰岛素敏感性及糖脂代谢的影响

Effect of Kusuan Tongtiao Decoction on Insulin Sensitibity and Glucolipid Metabolism in Type 2 Diabetes Rats

目的观察苦酸通调方对2型胰岛素抵抗大鼠胰岛素敏感性及糖脂代谢的影响。方法将高脂饲料喂养的雄性SPF级ZDF大鼠30只,随机分为模型组、苦酸通调组、吡格列酮组3组,普通饲料喂养的雄性SPF级ZL大鼠为正常对照组,每组各10只。苦酸通调方组按照3.29 g/(kg·d)灌胃,吡格列酮组按照1.07 mg/(kg·d)灌胃,模型组和正常对照组灌胃等体积蒸馏水,每日给药1次,连续给药12周。检测血糖(FBG)、糖化血红蛋白(Hb A1C)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、血清胰岛素(INS)等。结果与空白组ZL大鼠比较,模型组ZDF大鼠糖尿病状态明显,血清FBG、INS、Hb A1C、TC、TG、LDL-C、HDL-C水平升高(P<0.05)。与模型组比较,苦酸通调组血清FBG、INS、Hb A1C、TG、TC、LDL-C水平明显降低(P<0.05),但INS接近吡格列酮组(P>0.05)。结论苦酸通调方可以有效改善ZDF大鼠糖脂代谢及胰岛素抵抗,其改善2型糖尿病胰岛素抵抗可能是其防治糖尿病的机制之一。

Objective： To investigate the effect of Kusuan Tongtiao Decoction on insulin sensitibity and glucolipid metabolism in type 2 diabetes rats. Methods： 30 male Zucker rats（Zucker Diabetic Fatty rats） were induced by feeding of high-fat and high-carbohydrate diet were randomly divided into the model group（MD）,the Pioglitazone group（PG） and the Kusuan Tongtiao Decoction group（KG）. A normal control group（NG） was also set up by male Zucker Lean（ZL） rats which were feeding of normal diet.There were 10 rats in each group. The Kusuan Tongtiao Decoction group and the Pioglitazone group were given Kusuan Tongtiao Decoction 3.29g/（kg·d） and Pioglitazone suspension 1.07 mg/（kg·d） gavage respectively,while the normal group and model group were given the same amount of normal saline.After 12 weeks,all rats were weighed and fasting blood glucose（FBG）,fasting serum insulin（INS）,glycosylated hemoglobin（Hb A1c）,total cholesterol（TC）,triglyceride（TG）,low-density lipotrotein cholesterol（LDL-C） and high-density lipotrotein cholesterol（HDL-C） levels were compared between each group. Results： Compared with NG,there were obvious symptoms of diabetes in MG,levels of FBG,INS,Hb A1 C,TC,TG,LDL-C,HDL-C were significantly increased（P〈0.05）. Compared with MG,levels of FBG,INS,Hb A1 C,TG,TC,LDL-C were significantl decreased（P〈0.05）,but INS closed to PG. Conclusion： Kusuan Tongtiao Decoction can improve glucose and lipid metabolism and pancreatic islet function in type 2 diabetic rats. It can relieve insulin resistance that may be the possible mechanism of Kusuan Tongtiao Decoction preventing and controlling type 2 diabetes.