31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测

Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 31 Wild Core Sugarcane Germplasms

为明确甘蔗野生资源对黑顶柄锈菌的抗性水平,了解Bru1基因在甘蔗野生资源中的分布状况,于2013年对中国国家甘蔗种质资源圃保存的31份野生核心种质资源进行苗期抗褐锈病鉴定和抗褐锈病基因Bru1的分子检测。结果表明,31份供试材料中,高抗(1级)至中抗(3级)的有28份,占90.3%。其中19份材料表现高抗(1级),占61.3%,3份材料表现抗病(2级),占9.7%,6份材料表现中抗(3级),占19.4%。31份供试材料中只有贵州78-2-12、云南97-4、E.rockii95-19、E.rockii 95-20、云南83-224、广西79-8、云南95-35和广西89-13含抗褐锈病基因Bru1,占参试材料的25.8%;其余20份抗病材料和3份感病材料均不含抗褐锈病基因Bru1,表明除Bru1外,可能还有其他抗褐锈病基因存在。结果暗示中国国家甘蔗种质资源圃保存的野生核心种质资源中蕴藏着优良的抗褐锈病基因,是选育抗褐锈病甘蔗品种很有利用前景的抗源种质。

The objectives of this study was to clarify the level of resistance to Puccinia melanocephala and the distribution of Bru1 in sugarcane wild germplasm collections. Resistance of 31 wild core germplasm from National Nursery of Sugarcane Germplasm Resources（NNSGR, Kaiyuan, China） was identified at seedling stage, and the resistance gene Bru1 was detected in 2013. The results showed that 28 out of 31 materials（90.3%） were highly resistant（Grade 1） or moderately resistant（Grade 3）. Of them, nineteen（61.3%） were highly resistant（Grade 1）, three（9.7%） were resistant（Grade 2） and six（19.4%） were moderately resistant. Bru1 was detected in eight（25.8%） resistant materials（Guizhou 78-2-12, Yunnan 97-4, E. rockii 95-19, E. rockii 95-20, Yunnan 83-224, Guangxi 79-8, Yunnan 95-35, and Guangxi 89-13）. The absence of Bru1 in other 20 resistant and three susceptible entries suggests they may contain brown rust resistance-associated genes other than Bru1. Therefore the elite brown rust resistance gene should be contained in numerous sugarcane wild core germplasm collections of NNSGR, and be valuable for future brown rust resistant breeding programs in sugarcane.