高效液相色谱法测定复方丙睾凝胶剂中丙酸睾酮的含量

Determination of testosterone propionate in compound testosterone propionate gels by HPLC

目的：测定复方丙睾凝胶剂中丙酸睾酮的含量。方法：用高效液相色谱法,采用Kromasil C18柱,以甲醇-水（85∶15）为流动相,流速为0.8 ml/min,检测波长为254 nm,柱温为25℃。结果：丙酸睾酮在0.030 3~0.161 6 mg/ml内呈现良好的线性关系（r=0.999 9）。结论：本法可灵敏、快速、准确测定复方丙睾凝胶剂中丙酸睾酮的含量。

Objective ： To quantitative analysis of testosterone propionate in the compound testosterone propionate gels. Methods ： HPLC method was employed with a Kromasil Cls column used as stationary phase and methanol- water solution（85： 15 ） as mo- bile phase. The flow rate was 0.8 ml/min and the detection wavelength was at 254 nm. The temperature of the column was set at 25 ℃. Results ： The standard curve of testosterone propionate reference was well linear over the range of 0.030 3 - 0. 161 6 mg/ml （r = 0.999 9）. Conclusion ： This method is simple and fast, and the results are credible. HPLC method could be used as determi- nation the content of testosterone propionate in compound testosterone propionate Gels.